两种金化合物对溶酶体的作用。
Effect of two gold compounds on lysosomes.
作者信息
Graabaek P M, Pedersen S M
机构信息
Department of Anatomy, University of Aarhus, Denmark.
出版信息
Ann Rheum Dis. 1988 Jun;47(6):509-14. doi: 10.1136/ard.47.6.509.
The effect of two gold(I) compounds on stability of lysosomes in vitro was studied. Lysosomes from homogenates of rat kidney cortex were isolated by differential centrifugation. These lysosomes were incubated at 37 degrees C with widely varied concentrations of sodium aurothiomalate and sodium aurothiosulphate for 5, 35, and 65 minutes. Acid phosphatase activities were measured and used as an indication of lysosomal membrane stability in the presence and absence of drugs. The enhanced release of acid phosphatase from lysosomes by aurothiomalate and aurothiosulphate was related to dose, but the drugs differed substantially in their potencies. The disruptive effect on lysosomes was more marked for aurothiosulphate than for aurothiomalate. In addition, both drugs inhibited acid phosphatase activities at relatively high gold concentrations. Aurothiomalate had a moderate and aurothiosulphate a weaker inhibitory effect on the enzyme. Our results indicate that aurothiomalate and aurothiosulphate exert their beneficial effect in the treatment of rheumatoid arthritis through mechanism(s) other than lysosomal membrane stabilisation.
研究了两种一价金化合物对体外溶酶体稳定性的影响。通过差速离心从大鼠肾皮质匀浆中分离出溶酶体。将这些溶酶体在37℃下与浓度差异很大的硫代苹果酸金钠和硫代硫酸金钠孵育5、35和65分钟。测量酸性磷酸酶活性，并将其用作在有和没有药物存在的情况下溶酶体膜稳定性的指标。硫代苹果酸金钠和硫代硫酸金钠使溶酶体中酸性磷酸酶释放增加与剂量有关，但这两种药物的效力有很大差异。硫代硫酸金钠对溶酶体的破坏作用比硫代苹果酸金钠更明显。此外，在相对较高的金浓度下，两种药物均抑制酸性磷酸酶活性。硫代苹果酸金钠对该酶有中等抑制作用，硫代硫酸金钠的抑制作用较弱。我们的结果表明，硫代苹果酸金钠和硫代硫酸金钠在类风湿性关节炎治疗中发挥有益作用的机制并非通过溶酶体膜稳定化。
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