花生四烯酸可在无其他刺激的情况下，由纯化的人嗜酸性粒细胞诱导白三烯C4的形成。
Arachidonic acid can induce leukotriene C4 formation by purified human eosinophils in the absence of other stimuli.
作者信息
Kok P T, Hamelink M L, Kijne A M, Verhagen J, Koenderman L, Bruynzeel P L
机构信息
Department of Pulmonary Disease, State University Hospital Utrecht, The Netherlands.
出版信息
Biochem Biophys Res Commun. 1988 Jun 16;153(2):676-82. doi: 10.1016/s0006-291x(88)81148-3.
Stimulation of purified human eosinophils with 50 microM arachidonic acid leads to the production of leukotriene C4, 15-hydroxy-eicosatetraenoic acid and 15-series leukotrienes. The ratio of the amounts of leukotriene C4 and 15-lipoxygenase products was found to be strongly dependent on the arachidonic acid concentration, being relatively large at low arachidonic acid concentrations and very small at high arachidonic acid concentrations. In the presence of 1 microM platelet-activating factor a significant elevation of leukotriene C4 formation is observed, whereas the formation of 15-lipoxygenase products remains unaltered. As arachidonic acid was found to be capable of inducing a fast, transient rise in the cytosolic free Ca2+ concentration, this explains at least partly its ability to induce the Ca2+-dependent formation of leukotriene C4.
用50微摩尔花生四烯酸刺激纯化的人嗜酸性粒细胞会导致白三烯C4、15 - 羟基 - 二十碳四烯酸和15 - 系列白三烯的产生。发现白三烯C4与15 - 脂氧合酶产物的量之比强烈依赖于花生四烯酸浓度，在低花生四烯酸浓度下相对较大，而在高花生四烯酸浓度下非常小。在存在1微摩尔血小板活化因子的情况下，观察到白三烯C4形成显著升高，而15 - 脂氧合酶产物的形成保持不变。由于发现花生四烯酸能够诱导胞质游离Ca2 +浓度快速、短暂升高，这至少部分解释了其诱导白三烯C4依赖Ca2 +形成的能力。
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