Verhagen J, Bruynzeel P L, Koedam J A, Wassink G A, de Boer M, Terpstra G K, Kreukniet J, Veldink G A, Vliegenthart J F
FEBS Lett. 1984 Mar 12;168(1):23-8. doi: 10.1016/0014-5793(84)80199-4.
Human granulocytes isolated from peripheral blood have been described to synthesize both LTB4 and LTC4 from arachidonic acid. We have observed that the amount of LTC4 produced by human granulocyte preparations is strongly dependent on the relative amount of eosinophils. To investigate a possibly significant difference in leukotriene synthesis of the eosinophilic and neutrophilic granulocytes, we developed a purification method to isolate both cell types from granulocytes obtained from the blood of healthy donors. Leukotrienes were generated by incubation of the purified cells with arachidonic acid, calcium ionophore A23187, calcium-chloride and reduced glutathione. Surprisingly, eosinophils were found to produce almost exclusively the spasmogenic LTC4. In contrast, neutrophils produce almost exclusively the chemotactic LTB4, its omega-hydroxylated metabolite 20-hydroxy-LTB4 and two non-enzymically formed LTB4 isomers.
从外周血中分离出的人粒细胞已被描述为能从花生四烯酸合成白三烯B4（LTB4）和白三烯C4（LTC4）。我们观察到，人粒细胞制剂产生的LTC4量强烈依赖于嗜酸性粒细胞的相对数量。为了研究嗜酸性粒细胞和中性粒细胞在白三烯合成方面可能存在的显著差异，我们开发了一种纯化方法，从健康供体血液中获得的粒细胞中分离出这两种细胞类型。通过将纯化的细胞与花生四烯酸、钙离子载体A23187、氯化钙和还原型谷胱甘肽一起孵育来生成白三烯。令人惊讶的是，发现嗜酸性粒细胞几乎只产生致痉挛的LTC4。相比之下，中性粒细胞几乎只产生趋化性的LTB4、其ω-羟基化代谢产物20-羟基-LTB4以及两种非酶促形成的LTB4异构体。
