Emoto N, Baird A
Laboratories for Neuroendocrinology, Salk Institute, La Jolla, CA 92037.
Biochem Biophys Res Commun. 1988 Jun 16;153(2):792-8. doi: 10.1016/s0006-291x(88)81165-3.
We investigated the effects of tumor necrosis factor (TNF)/cachectin on follicle-stimulating hormone (FSH)-induced aromatase activity in cultured rat granulosa cells using the stereospecific transfer of 3H from [1 beta-3H] androstenedione into 3H2O. TNF (10 pg/ml-10 ng/ml) inhibited FSH (250 ng/ml)-induced aromatase activity in a concentration-dependent manner, and 10 ng/ml of TNF completely abolished the FSH-induced aromatase activity. A time course analysis of the effects of TNF showed that TNF had no effect on induced aromatase activity, but inhibited the further induction of the enzyme by FSH. TNF (10 ng/ml) also inhibited the ability of TGF beta (1 ng/ml) to enhance aromatase activity and increase progesterone synthesis. Thus, TNF is a component of the complex array of proteins that modulate ovarian function and, as such, may play a physiological role in the regulation of the granulosa cell. In view of its association with cachexia, it may also play a pathophysiological role in the suppression of reproductive function during chronic illness.
我们使用[1β-3H]雄烯二酮中3H向3H2O的立体特异性转移，研究了肿瘤坏死因子（TNF）/恶病质素对培养的大鼠颗粒细胞中促卵泡激素（FSH）诱导的芳香化酶活性的影响。TNF（10 pg/ml - 10 ng/ml）以浓度依赖的方式抑制FSH（250 ng/ml）诱导的芳香化酶活性，10 ng/ml的TNF完全消除了FSH诱导的芳香化酶活性。TNF作用的时间进程分析表明，TNF对诱导的芳香化酶活性没有影响，但抑制了FSH对该酶的进一步诱导。TNF（10 ng/ml）还抑制了转化生长因子β（1 ng/ml）增强芳香化酶活性和增加孕酮合成的能力。因此，TNF是调节卵巢功能的复杂蛋白质阵列的一个组成部分，因此可能在颗粒细胞的调节中发挥生理作用。鉴于其与恶病质的关联，它也可能在慢性疾病期间生殖功能的抑制中发挥病理生理作用。
