Brosalina E B, Vlasov V V, Kazakov S A
Bioorg Khim. 1988 Jan;14(1):125-8.
A one-tube synthesis scheme for coupling EDTA residue to the 5'-terminus of unprotected oligonucleotides via propylenediamine linker is described. The EDTA derivative of oligonucleotide d(pTGACCCTCTTCCC)A forms a kinetically stable complex with Fe2+ ion. In the presence of ascorbic acid with O2 limiting, this complex modifies single-stranded DNA (a 302-mer) in a site-specific way near the target complementary nucleotide sequence. The DNA fragment can be then cleaved predominantly at modified pyrimidine nucleotides (hidden breaks) by hot piperidine treatment, whereas few direct breaks (i.e. without piperidine) occurs at this site. No autocleavage of the [Fe.EDTA]-oligonucleotide derivative is observed under the experiment's conditions.
描述了一种通过丙二胺连接子将EDTA残基偶联到未保护寡核苷酸5'-末端的单管合成方案。寡核苷酸d(pTGACCCTCTTCCC)A的EDTA衍生物与Fe2+离子形成动力学稳定的复合物。在抗坏血酸存在且氧气受限的情况下，该复合物以位点特异性方式在靶互补核苷酸序列附近修饰单链DNA（302聚体）。然后通过热哌啶处理，DNA片段主要在修饰的嘧啶核苷酸处被切割（隐蔽切割），而在此位点很少发生直接切割（即无哌啶存在时）。在实验条件下未观察到[Fe.EDTA]-寡核苷酸衍生物的自切割现象。
