Center of Excellence for Indigenous Biological Resources-Genome Studies, Faculty of Mathematics and Natural Sciences, Universitas Indonesia, Kampus UI Depok, 16424, Indonesia.
Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Indonesia, Kampus UI Depok, 16424, Indonesia.
Int J Syst Evol Microbiol. 2019 Oct;69(10):3080-3086. doi: 10.1099/ijsem.0.003594.
Six mycelium-forming actinomycete strains were isolated from forest soil near the Cisolok geysers in West Java, Indonesia. The 16S rRNA gene sequences of these strains showed high similarity to members of genera in the family with values less than 96.0 %, and most closely related to the genus AG2-7(94.6-95.2 % similarity). The type strain, designated SL3-2-4, was aerobic, thermophilic, Gram-stain-positive that formed branched, non-fragmented substrate mycelia and unbranched aerial mycelia with long-chain, oval-shaped spores on International Project (ISP) 3 medium. It produced light-orange substrate mycelia and light-orange diffusible pigments on ISP 3 medium with 2 % gellan gum, grown at 30-55 °C, with optimum growth at 45 °C. The pH range for growth was 4.0-8.0, with optimum growth at pH 7.0. Strain SL3-2-4 was able to hydrolyze casein, esculin, gelatin, guanine, hypoxanthine, starch, L-tyrosine, and xanthine, but not adenine, carboxymethyl-cellulose, cellulose, chitin, Tween 20, or xylan. The major fatty acid was -C, and the major menaquinone was MK-8 (H). The detected polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidyl--methylethanolamine, unidentified aminophospholipids, unidentified glycolipids, and unidentified phospholipids. The cell wall hydrolysate of SL3-2-4 contained -2,4-diaminopimelic acid. The whole cell sugars were arabinose and galactose. The DNA G+C content was 71.6 mol%. Phenotypic features and phylogenetic data differentiated SL3-2-4 from members of the family Therefore, the strain SL3-2-4 is proposed as a representative of a novel species in a novel genus, gen. nov., sp. nov. The type strain is SL3-2-4 (=UICC B-83=NRRL B-67478=InaCC A981).
从印度尼西亚西爪哇的 Cisolok 间歇泉附近的森林土壤中分离到了 6 株形成菌丝的放线菌菌株。这些菌株的 16S rRNA 基因序列与 科的属成员具有高相似性，相似度值均小于 96.0%，与 属最为接近，相似度为 94.6-95.2%。模式菌株 SL3-2-4 为需氧、嗜热、革兰氏阳性，形成分支、不分枝的基质菌丝和无分支的气生菌丝，在国际项目（ISP）3 培养基上产生长链、椭圆形孢子。它在 ISP3 培养基上产生浅橙色的基质菌丝和浅橙色可扩散色素，在 2%卡拉胶中生长，生长温度为 30-55°C，最适生长温度为 45°C。生长的 pH 值范围为 4.0-8.0，最适生长 pH 值为 7.0。菌株 SL3-2-4 能够水解酪蛋白、七叶灵、明胶、鸟嘌呤、次黄嘌呤、淀粉、L-酪氨酸和黄嘌呤，但不能水解腺嘌呤、羧甲基纤维素、纤维素、几丁质、吐温 20 或木聚糖。主要脂肪酸为 -C，主要的menaquinone 为 MK-8 (H)。检测到的极性脂类有双磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺、磷脂酰--甲乙醇胺、未鉴定的氨基磷脂、未鉴定的糖脂和未鉴定的磷脂。SL3-2-4 的细胞壁水解产物含有 -2,4-二氨基庚二酸。全细胞糖为阿拉伯糖和半乳糖。DNA G+C 含量为 71.6 mol%。表型特征和系统发育数据将 SL3-2-4 与 科的成员区分开来。因此，菌株 SL3-2-4 是一个新属、新种的代表，新属名为 ，新种名为 。模式菌株为 SL3-2-4（=UICC B-83=NRRL B-67478=InaCC A981）。
