Delers F, Strecker G, Engler R
Université René-Descartes, Faculté de médecine des Saints-Pères, Laboratoire des protéines de la réaction inflammatoire, Paris, France.
Biochem Cell Biol. 1988 Mar;66(3):208-17. doi: 10.1139/o88-028.
Chicken haptoglobin (Hp), a hemoglobin-binding protein isolated from chicken plasma, is composed of three molecular variants that react differently with concanavalin A (ConA). These glycosylation variants of chicken Hp have been isolated by affinity chromatography using Sepharose-bound ConA. They differ in their molecular weight, as determined by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. Analysis of the glycopeptides obtained after pronase digestion of these variants yielded two types of structures: one, reactive with ConA, corresponded to a biantennary N-linked carbohydrate unit and one, unreactive with ConA, corresponded to a triantennary unit. The strongly ConA-reactive Hp variant bears only two biantennary units and the nonreactive Hp variant bears only two triantennary units; the weakly reactive Hp variant bears equal amounts of both units. The distribution of Hp glycosylation variant does not show any significant difference when obtained from the plasma of laying hens before and after turpentine-induced inflammation.
鸡触珠蛋白（Hp）是一种从鸡血浆中分离出来的血红蛋白结合蛋白，由三种分子变体组成，它们与伴刀豆球蛋白A（ConA）的反应不同。鸡Hp的这些糖基化变体已通过使用琼脂糖结合的ConA的亲和色谱法分离出来。通过在十二烷基硫酸钠存在下的聚丙烯酰胺凝胶电泳测定，它们的分子量不同。对这些变体经链霉蛋白酶消化后得到的糖肽分析产生了两种类型的结构：一种与ConA反应，对应于一个双天线N-连接碳水化合物单元；另一种与ConA不反应，对应于一个三天线单元。与ConA强烈反应的Hp变体仅带有两个双天线单元，不反应的Hp变体仅带有两个三天线单元；反应较弱的Hp变体带有等量的这两种单元。从产蛋母鸡在松节油诱导炎症前后的血浆中获得的Hp糖基化变体分布没有显示出任何显著差异。
