Institute of Vegetable Research, Hunan Academy of Agricultural Sciences, Changsha, China.
Institute of Plant Protection, Hunan Academy of Agricultural Sciences, Changsha, China.
PLoS One. 2019 Jul 22;14(7):e0219775. doi: 10.1371/journal.pone.0219775. eCollection 2019.
WRKY proteins are a large group of plant transcription factors that are involved in various biological processes, including biotic and abiotic stress responses, hormone response, plant development, and metabolism. WRKY proteins have been identified in several plants, but only a few have been identified in Capsicum annuum. Here, we identified a total of 62 WRKY genes in the latest pepper genome. These genes were classified into three groups (Groups 1-3) based on the structural features of their proteins. The structures of the encoded proteins, evolution, and expression under normal growth conditions were analyzed and 35 putative miRNA target sites were predicted in 20 CaWRKY genes. Moreover, the response to cold or CMV treatments of selected WRKY genes were examined to validate the roles under stresses. And alternative splicing (AS) events of some CaWRKYs were also identified under CMV infection. Promoter analysis confirmed that CaWRKY genes are involved in growth, development, and biotic or abiotic stress responses in hot pepper. The comprehensive analysis provides fundamental information for better understanding of the signaling pathways involved in the WRKY-mediated regulation of developmental processes, as well as biotic and abiotic stress responses.
WRKY 蛋白是一大类植物转录因子，参与多种生物学过程，包括生物和非生物胁迫响应、激素响应、植物发育和代谢。WRKY 蛋白已在几种植物中被鉴定出来，但在辣椒中只鉴定出少数几种。在这里，我们在最新的辣椒基因组中总共鉴定出 62 个 WRKY 基因。这些基因根据其蛋白质的结构特征分为三组（第 1-3 组）。分析了编码蛋白的结构、进化以及在正常生长条件下的表达，并在 20 个 CaWRKY 基因中预测了 35 个假定的 miRNA 靶位。此外，还检测了选定的 WRKY 基因对冷或 CMV 处理的反应，以验证在胁迫下的作用。还鉴定了一些 CaWRKY 在 CMV 感染下的选择性剪接（AS）事件。启动子分析证实，CaWRKY 基因参与了辣椒的生长、发育以及生物或非生物胁迫反应。综合分析为更好地理解 WRKY 介导的发育过程以及生物和非生物胁迫响应的信号通路提供了基础信息。
