在鸡胚中注射益生元和合生元对肉鸡胸浅肌生产性能和微观结构特征的影响。

Effects of in ovo injection of prebiotics and synbiotics on the productive performance and microstructural features of the superficial pectoral muscle in broiler chickens.

机构信息

Department of Animal Physiology, Physiotherapy and Nutrition, Poland.

Department of Agricultural, Environmental and Food Sciences, University of Molise; 86100 Campobasso, Italy.

出版信息

Poult Sci. 2019 Oct 1;98(10):5157-5165. doi: 10.3382/ps/pez202.

DOI:10.3382/ps/pez202

PMID:31329998

Abstract

The aim of the study was to compare the effects of 2 prebiotics and 2 synbiotics injected in ovo on productivity parameters, quality, and microstructure of the superficial pectoral muscle in 35-day-old broiler chickens. On day 12 of incubation, 9,000 eggs Ross 308 were randomly divided into 5 experimental groups treated with different bioactives in ovo injected: C, control with physiological saline; PI, with 1.760 mg inulin; PB, with 0.528 mg of commercial prebiotic Bi2tos; SI, with 1.760 mg inulin and 1,000 CFU Lactococcus lactis spp. lactis IBB SL1; SB, with 0.528 mg Bi2tos and 1,000 CFU Lactococcus lactis spp. cremoris IBB SC1. The synbiotic solution contained 20 μl bacterial suspension and 180 μl prebiotic solution. For productive parameters and further tests ten male birds for each experimental group were used. The birds were slaughtered on day 35 of age. At slaughter, samples of the left pectoral muscles were taken and preserved by freezing in liquid nitrogen. The pH and color of the meat were evaluated at 45 min and 24 h post-mortem. Water holding capacity (WHC) was measured and expressed as the percentage of free water in meat. Microscopic specimens were analysed using MultiScan software for the measurement of the percentage of oxidative and glycolytic fibres and mean diameter of the muscle fibres. In ovo injection of prebiotics Bi2tos had a positive effect on body weight. In prebiotic group (PI) a negative impact on hatchability was observed. Prebiotics and synbiotics had no influence on the yield of the carcass and pectoral muscle. Bioactive compounds had a significant effect on the quality of meat parameters such as: pH 24 h (PI and PB group), L* 45' (SI and SB group), and WHC (groups PB, SI, and SB). The analysis of the enzymatic profile showed a significant increase in the percentage of glycolytic fibres in the pectoral muscle from chicken treated with a synbiotic with the addition of inulin (group SI).

摘要

本研究旨在比较在卵内注射两种益生元和两种合生剂对 35 日龄肉鸡生产性能参数、肉质和胸肌微观结构的影响。在孵化第 12 天，将 9000 枚罗斯 308 蛋随机分为 5 个实验组，用不同的卵内生物活性物质处理：C 组，用生理盐水；PI 组，用 1.760mg 菊粉；PB 组，用 0.528mg 商业益生元 Bi2tos；SI 组，用 1.760mg 菊粉和 1000CFU 乳酸乳球菌 spp. lactis IBB SL1；SB 组，用 0.528mg Bi2tos 和 1000CFU 乳酸乳球菌 spp. cremoris IBB SC1。合生剂溶液含有 20μl 细菌悬浮液和 180μl 益生元溶液。为了进行生产性能参数和进一步的测试，每个实验组使用 10 只雄性鸟类。在 35 日龄时进行屠宰。屠宰时，取左侧胸肌样本，用液氮冷冻保存。在宰后 45 分钟和 24 小时测量肉的 pH 值和颜色。测量持水力（WHC），并表示为肉中游离水的百分比。使用 MultiScan 软件分析显微镜标本，测量氧化和糖酵解纤维的百分比和肌纤维的平均直径。在卵内注射益生元 Bi2tos 对体重有积极影响。在益生元组（PI）中，孵化率受到负面影响。益生元和合生剂对胴体和胸肌的产率没有影响。生物活性化合物对肉品质参数有显著影响，如：pH24 小时（PI 和 PB 组）、L*45'（SI 和 SB 组）和 WHC（PB、SI 和 SB 组）。酶谱分析显示，添加菊粉的合生剂处理的鸡胸肌中糖酵解纤维的百分比显著增加（SI 组）。