Bogucka Joanna, Dankowiakowska Agata, Elminowska-Wenda Gabriela, Sobolewska Adrianna, Szczerba Agata, Bednarczyk Marek
Folia Biol (Krakow). 2016;64(3):131-143. doi: 10.3409/fb64_3.131.
The objective of the study was to determine the effect of prebiotics and synbiotics administered in ovo on the 12th day of incubation, on the development of the intestinal villi and the number of goblet cells in the small intestine of broiler chickens on the Ist and the 4th days of life of chicks. Two prebiotics: inulin (PI) or Bi2tos (PB) and two synbiotics: inulin + L. lactis subsp. lactis (SI) or Bi2tos + L. lactis subsp. cremoris (SB) were injected in ovo on the 12th day of embryonic development. The control group of the embryos was injected with physiological saline (C). On the 1st day of life, an increase in the height of the villi in the jejunum was reported as a result of the injection of pre- and synbiotics, moreover an increase in the surface area of the villi in the jejunum and the duodenum in chicks from the SB group was also observed. A stimulatory effect of synbiotics on the morphology of the duodenum and thejejunum was also observed on the 4th dh day after hatching. Conversely, in the ileum, in the SB group, a reduction in the height of villi was found both on thse 1t and thte 4h days of life. In contrast, injection of inulin and synbiotic with the addition of inulin resulted in an increase in the number of goblet cells in the duodenum and the jejunum on thst 1" day of life, and caused a significant decrease on the 4th day after hatching.
本研究的目的是确定在孵化第12天进行卵内注射益生元和合生元，对雏鸡出生第1天和第4天小肠绒毛发育及小肠杯状细胞数量的影响。两种益生元：菊粉（PI）或Bi2tos（PB），以及两种合生元：菊粉 + 乳酸乳球菌乳酸亚种（SI）或Bi2tos + 乳酸乳球菌乳脂亚种（SB），在胚胎发育第12天进行卵内注射。胚胎对照组注射生理盐水（C）。在雏鸡出生第1天，报告称注射益生元和合生元后空肠绒毛高度增加，此外还观察到SB组雏鸡空肠和十二指肠绒毛表面积增加。在孵化后第4天也观察到合生元对十二指肠和空肠形态有刺激作用。相反，在回肠，SB组在出生第1天和第4天均发现绒毛高度降低。相比之下，注射菊粉以及添加菊粉的合生元，在雏鸡出生第1天导致十二指肠和空肠杯状细胞数量增加，而在孵化后第4天则导致显著减少。
