Department of Molecular Biology, School of Pharmacy with Division of Laboratory Medicine in Sosnowiec, Medical University of Silesia in Katowice, Katowice, Poland.
Department of Cosmetology, School of Pharmacy with Division of Laboratory Medicine in Sosnowiec, Medical University of Silesia in Katowice, Katowice, Poland.
Dermatol Ther. 2019 Sep;32(5):e13029. doi: 10.1111/dth.13029. Epub 2019 Jul 22.
The aim of this study was to assess changes in the expression of STAT1, STAT3, STAT4, SOCS2, and IL17 in psoriatic patients under ustekinumab treatment and in healthy volunteers. The study group consisted of 14 patients suffering from psoriasis vulgaris qualified for ustekinumab therapy (4 women, 10 men) The control group consisted of 14 healthy volunteers (7 women, 7 men), their whole blood was used as a material in this study. A quantitative reverse transcription polymerase chain assay was used to amplify analyzed genes. To indicate the differences in expression of selected genes in the test and control groups, the Kruskal-Wallis and the post hoc Dunn's test was carried out. After 40 weeks of observation of the effectiveness of ustekinumab in patients with psoriasis, the expression of STAT1, STAT3, STAT4, IL17, and SOCS2 was silenced. Statistic differences in expression were observed for STAT3 (40 vs. 0 weeks, p < .05; 0 week vs. C, p < .05) and SOCS2 (0 week vs. C, p < .05). Patients with psoriasis vulgaris have higher levels of STAT1, STAT3, STAT4, SOCS2, and IL17 expression compared to healthy individuals. On the other hand, the treatment of ustekinumab lasting 40 weeks caused a decrease in the transcriptional activity of the analyzed genes.
本研究旨在评估在接受乌司奴单抗治疗的银屑病患者和健康志愿者中，STAT1、STAT3、STAT4、SOCS2 和 IL17 的表达变化。研究组包括 14 名符合乌司奴单抗治疗条件的寻常型银屑病患者（4 名女性，10 名男性），对照组包括 14 名健康志愿者（7 名女性，7 名男性），他们的全血被用作本研究的材料。采用定量逆转录聚合酶链反应（qRT-PCR）扩增分析基因。为了表明所选基因在试验组和对照组中的表达差异，进行了 Kruskal-Wallis 和事后 Dunn 检验。在观察银屑病患者乌司奴单抗治疗 40 周后的疗效后，STAT1、STAT3、STAT4、IL17 和 SOCS2 的表达被沉默。在 STAT3（40 周与 0 周相比，p<.05；0 周与 C 相比，p<.05）和 SOCS2（0 周与 C 相比，p<.05）中观察到表达差异的统计学意义。与健康个体相比，寻常型银屑病患者的 STAT1、STAT3、STAT4、SOCS2 和 IL17 表达水平更高。另一方面，乌司奴单抗治疗 40 周导致分析基因的转录活性降低。
