大黄鱼鱼鳔胶原蛋白的理化性质、抗氧化特性及其创伤愈合评价。

Physicochemical, antioxidant properties of giant croaker (Nibea japonica) swim bladders collagen and wound healing evaluation.

机构信息

Zhejiang Provincial Engineering Technology Research Center of Marine Biomedical Products, School of Food and Pharmacy, Zhejiang Ocean University, Zhoushan 316022, China.

Hangzhou Obstetrics & Gynecology Hospital, Hangzhou 310008, China.

出版信息

Int J Biol Macromol. 2019 Oct 1;138:483-491. doi: 10.1016/j.ijbiomac.2019.07.111. Epub 2019 Jul 19.

DOI:10.1016/j.ijbiomac.2019.07.111

PMID:31330209

Abstract

Acid-solubilized collagen (ASC) and pepsin-solubilized collagen (PSC) were obtained from Nibea japonica swim bladders. The denaturation temperature (Td) of ASC and PSC was approximately 33.8 °C. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Fourier transform infrared spectroscopy (FTIR) analyses indicated that ASC and PSC contained triple-helical type I collagen when compared to rat tail collagen type I. Moreover, the microstructure of collagen sponges was uniform and porous. In addition, ASC and PSC exhibited antioxidant properties and in vitro scratch assays showed that PSC at various concentrations (0, 12.5, 25, and 50 μg/mL) had significant effects on the scratch closure rate. Furthermore, collagen sponge from Nibea japonica swim bladders exhibited an increased efficacy of wound healing when compared to the control mice. The levels of interleukin (IL)-1β, IL-6 and tumor necrosis factor (TNF)-α in the collagen sponge treated mice were significantly decreased when compared to the control group. Thus, our results suggested that collagen sponge from Nibea japonica swim bladders has potential wound healing applications.

摘要

酸溶性胶原蛋白（ASC）和胃蛋白酶溶性胶原蛋白（PSC）从日本红娘鱼鱼鳔中提取得到。ASC 和 PSC 的变性温度（Td）约为 33.8°C。与大鼠尾腱 I 型胶原蛋白相比，十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）和傅立叶变换红外光谱（FTIR）分析表明 ASC 和 PSC 均含有三螺旋 I 型胶原蛋白。此外，胶原蛋白海绵的微观结构均匀且多孔。另外，ASC 和 PSC 表现出抗氧化特性，体外划痕实验表明，不同浓度（0、12.5、25 和 50μg/mL）的 PSC 对划痕闭合率有显著影响。此外，与对照组相比，日本红娘鱼鱼鳔胶原蛋白海绵在促进伤口愈合方面的效果明显增强。与对照组相比，在胶原蛋白海绵处理的小鼠中，白细胞介素（IL）-1β、IL-6 和肿瘤坏死因子（TNF）-α的水平显著降低。因此，我们的结果表明，日本红娘鱼鱼鳔胶原蛋白海绵具有潜在的伤口愈合应用价值。