State Key Laboratory of Pollution Control and Resources Reuse, College of Environmental Science and Engineering, Tongji University, 200092, Shanghai, China.
State Key Laboratory of Pollution Control and Resources Reuse, College of Environmental Science and Engineering, Tongji University, 200092, Shanghai, China.
J Hazard Mater. 2019 Dec 15;380:120877. doi: 10.1016/j.jhazmat.2019.120877. Epub 2019 Jul 12.
In recent years, photocatalysis has been considered as a promising method, which provides measures to environmental pollution. Antibiotic resistant bacteria (ARB) and their antibiotic resistance genes (ARGs), as the emerging environmental pollutants, are released into the environment, resulting in antibiotic resistance spread. TiO-based nanocomposites, as the most common photocatalytic material, may influence ARB and ARGs under photocatalytic conditions. However, the research on this aspect is rare. A novel nanocomposite synthesized from Ag, TiO and graphene oxide (GO), was selected as a representative of nanomaterials for investigation. The experimental results indicated that TiO/Ag/GO nanocomposites significantly affected ARB vitality. 100 mg/L TiO/Ag/GO will reduce bacterial survival to 12.2% in 10 min under simulated sunlight irradiation. Chloramphenicol as the most representative antibiotic in the water, reduces the effect of ARB inactivation under photocatalytic conditions. The addition of TiO/Ag/GO could affect tetracycline antibiotic resistance. The level of bacterial tolerance to tetracycline had a significant reduction. The horizontal gene transfer was promoted from 1 to 2 folds with the addition of TiO/Ag/GO. Even high TiO/Ag/GO concentration (100 mg/L) sample had a limited promotion, suggesting that TiO/Ag/GO will not increase the risk of antibiotic resistance spread compared to other nano materials.
近年来，光催化被认为是一种很有前途的方法，可以为环境污染提供措施。抗生素耐药细菌（ARB）及其抗生素耐药基因（ARGs）作为新兴的环境污染物，被释放到环境中，导致抗生素耐药性的传播。基于 TiO 的纳米复合材料作为最常见的光催化材料，在光催化条件下可能会影响 ARB 和 ARGs。然而，这方面的研究很少。一种由 Ag、TiO 和氧化石墨烯（GO）合成的新型纳米复合材料被选为纳米材料的代表进行研究。实验结果表明，TiO/Ag/GO 纳米复合材料显著影响 ARB 的活力。在模拟阳光照射下，100mg/L 的 TiO/Ag/GO 在 10min 内将细菌存活率降低到 12.2%。氯霉素作为水中最具代表性的抗生素，降低了光催化条件下 ARB 失活的效果。添加 TiO/Ag/GO 会影响四环素类抗生素的耐药性。细菌对四环素的耐受性水平显著降低。随着 TiO/Ag/GO 的添加，水平基因转移从 1 倍增加到 2 倍。即使添加高浓度的 TiO/Ag/GO（100mg/L）也有有限的促进作用，这表明与其他纳米材料相比，TiO/Ag/GO 不会增加抗生素耐药性传播的风险。
