Institute of Materials Science and Technology, Sichuan University, Chengdu, 610065, China.
School of Material Science and Chemical Engineering, Tianjin Universuty of Science & Technology, Tianjin, 300000, China.
Colloids Surf B Biointerfaces. 2019 Oct 1;182:110347. doi: 10.1016/j.colsurfb.2019.110347. Epub 2019 Jul 8.
Stimuli-responsive smart polymers have been studied extensively. In this work, thermoresponsive poly (N-isopropylacrylamide-N-methylolacrylamide-acrylamide) (PNIPAm-NMA-Am) was successfully synthesised via radical polymerisation, as confirmed by proton nuclear magnetic resonance and fourier transform infrared spectroscopy. PNIPAm-NMA-Am was electrospun into nanofibres, allowing its use as a drug carrier after simple thermal treatment. Thermogravimetric analysis, scanning electron microscopy, and atomic force microscopy results also revealed that the as-prepared PNIPAm-NMA-Am nanofibres have a uniform small diameter, good thermal stability and excellent integrity in aqueous environments. Additionally, the properties of this PNIPAm-NMA-Am nanofibres were tunable with temperature changes below and above the lower critical solution temperature of 48 °C. The drug release properties of PNIPAm-NMA-Am10 nanofibres as a drug carrier were studied via ultraviolet-visible spectroscopy and the results showed that 80% of the drug was released from the nanofibres after six heating and cooling (60-10 °C) cycles within 60 min. Only a small amount of the drug was released during the cooling process, which directly demonstrates "on-off" functionality of PNIPAm-NMA-Am nanofibres for controlled drug release. Finally, cell culture studies indicated that the PNIPAm-NMA-Am nanofibres have not cytotoxicity. Thus, the novel PNIPAm-NMA-Am nanofibres show great potential in the biomedical field as drug carriers.
刺激响应型智能聚合物已得到广泛研究。在这项工作中，通过自由基聚合成功合成了温敏性聚（N-异丙基丙烯酰胺-N-羟甲基丙烯酰胺-丙烯酰胺）（PNIPAm-NMA-Am），这通过质子核磁共振和傅里叶变换红外光谱得到了证实。PNIPAm-NMA-Am 被电纺成纳米纤维，经过简单的热处理后可用作药物载体。热重分析、扫描电子显微镜和原子力显微镜的结果还表明，所制备的 PNIPAm-NMA-Am 纳米纤维具有均匀的小直径、良好的热稳定性和在水相环境中的优异完整性。此外，这种 PNIPAm-NMA-Am 纳米纤维的性能可以通过低于和高于 48°C 的下临界溶液温度的温度变化进行调节。通过紫外可见光谱研究了 PNIPAm-NMA-Am10 纳米纤维作为药物载体的药物释放性能，结果表明，在 60 分钟内，经过 6 个加热和冷却（60-10°C）循环后，80%的药物从纳米纤维中释放出来。在冷却过程中只有少量药物释放，这直接证明了 PNIPAm-NMA-Am 纳米纤维对控制药物释放的“开-关”功能。最后，细胞培养研究表明，PNIPAm-NMA-Am 纳米纤维没有细胞毒性。因此，新型 PNIPAm-NMA-Am 纳米纤维在作为药物载体的生物医学领域具有巨大的潜力。
