Grupo de Investigación en Simulación, Diseño, Control y Optimización de Procesos (SIDCOP), Departamento de Ingeniería Química, Universidad de Antioquia, Medellín 050010, Colombia.
Grupo de Bioprocesos, Departamento de Ingeniería Química, Universidad de Antioquia, Medellín 050010, Colombia.
Int J Mol Sci. 2024 Oct 12;25(20):10992. doi: 10.3390/ijms252010992.
is a species used worldwide to industrially produce clavulanic acid (CA), a molecule that enhances antibiotic effectiveness against β-lactamase-producing bacterial strains. Despite its low inherent CA production, hyper-producing strains have been developed. However, genomic analyses specific to and CA biosynthesis are limited. Genomic variations that may influence CA yield were explored using strain genomes from diverse sources. Despite the slight differences obtained by similarity index calculation, pan-genome estimation revealed that only half of the genes identified were present in all strains. As expected, core genes were associated with primary metabolism, while the remaining genes were linked to secondary metabolism. Differences at the sequence level were more likely to be found in regions close to the tips of the linear chromosome. Wild-type strains preserved larger chromosomal and plasmid regions compared to industrial and/or hyper-producing strains; such a grouping pattern was also found through refined phylogenetic analyses. These results provide essential insights for the development of hyper-producing strains, attending to the critical demand for this antibiotic enhancer and contributing to future strategies for CA production optimization.
是一种在全球范围内被用于工业生产克拉维酸（CA）的物种，CA 是一种增强抗生素对产β-内酰胺酶细菌菌株有效性的分子。尽管其自身 CA 产量较低，但已经开发出了高产菌株。然而，针对 和 CA 生物合成的基因组分析是有限的。使用来自不同来源的 菌株基因组探索了可能影响 CA 产量的基因组变异。尽管通过相似度指数计算获得了微小差异，但泛基因组估计表明，并非所有菌株都存在鉴定出的一半基因。正如预期的那样，核心基因与初级代谢有关，而其余基因与次级代谢有关。在序列水平上的差异更可能出现在线性染色体尖端附近的区域。与工业和/或高产菌株相比，野生型菌株保留了更大的染色体和质粒区域；通过精细的系统发育分析也发现了这种分组模式。这些结果为开发高产 菌株提供了重要的见解，满足了对这种抗生素增强剂的关键需求，并为未来的 CA 生产优化策略做出了贡献。
