Faculty of Agriculture, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan.
Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan.
Mar Drugs. 2019 Jul 19;17(7):421. doi: 10.3390/md17070421.
Low molecular weight fucoidan extract (LMF), prepared by an abalone glycosidase digestion of a crude fucoidan extracted from Kylin, exhibits various biological activities, including anticancer effect. Various cancers express programmed cell death-ligand 1 (PD-L1), which is known to play a significant role in evasion of the host immune surveillance system. PD-L1 is also expressed in many types of normal cells for self-protection. Previous research has revealed that selective inhibition of PD-L1 expressed in cancer cells is critical for successful cancer eradication. In the present study, we analyzed whether LMF could regulate PD-L1 expression in HT1080 fibrosarcoma cells. Our results demonstrated that LMF suppressed PD-L1/PD-L2 expression and the growth of HT1080 cancer cells and had no effect on the growth of normal TIG-1 cells. Thus, LMF differentially regulates PD-L1 expression in normal and cancer cells and could serve as an alternative complementary agent for treatment of cancers with high PD-L1 expression.
低分子量褐藻糖胶提取物（LMF）是通过鲍鱼糖苷酶消化从麒麟中提取的粗褐藻糖胶制备而成的，具有多种生物活性，包括抗癌作用。各种癌症都表达程序性细胞死亡配体 1（PD-L1），PD-L1 已知在逃避宿主免疫监视系统中发挥重要作用。PD-L1 也在许多类型的正常细胞中表达以进行自我保护。先前的研究表明，选择性抑制癌细胞中表达的 PD-L1 对于成功消除癌症至关重要。在本研究中，我们分析了 LMF 是否可以调节 HT1080 纤维肉瘤细胞中的 PD-L1 表达。我们的结果表明，LMF 抑制 PD-L1/PD-L2 表达和 HT1080 癌细胞的生长，对正常 TIG-1 细胞的生长没有影响。因此，LMF 可在正常和癌细胞中差异调节 PD-L1 的表达，可作为治疗高 PD-L1 表达癌症的替代补充剂。
