Centre for Biotechnology and Functional Food, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, Malaysia.
University of Natural Resources and Life Sciences, Vienna, Department of Chemistry, Division of Chemistry of Renewable Resources, Muthgasse 18, A-1190 Vienna, Austria.
Food Chem. 2019 Jan 30;272:222-226. doi: 10.1016/j.foodchem.2018.08.034. Epub 2018 Aug 9.
Fucoidan is a sulphated polysaccharide, made up mainly of l-fucose, which is found in brown seaweeds. Its chemical structure is diverse and depends on maturity, species and geographical location. The objective of this study was to elucidate the chemical structure of fucoidan from Cladosiphon okamuranus harvested in Japan. The fucoidan was subject to purification prior to monosaccharide profiling, sulphate content determination, and linkage analysis. Our results showed that Japanese Cladosiphon okamuranus fucoidan contained 70.13 ± 0.22 wt% fucose and 15.16 ± 1.17 wt% sulphate. Other minor monosaccharides found were d-xylose, d-galactose, d-mannose, d-glucose, d-arabinose, d-rhamnose and d-glucuronic acid. Linkage analysis revealed that fucopyranoside units along the backbone are linked, through α-1,3-glycosidic bonds, with fucose branching at C-2, and one sulphate group at C-4 per every three fucose units, i.e. the structure of fucoidan from Japanese Cladosiphon okamuranus is [→3)-α-fuc(1→][→3)-α-fuc-4-OSO-(1→][→2)-α-fuc].
岩藻聚糖是一种硫酸化多糖，主要由岩藻糖组成，存在于褐藻中。其化学结构多样，取决于成熟度、物种和地理位置。本研究旨在阐明日本采集的角叉菜岩藻聚糖的化学结构。岩藻聚糖在进行单糖分析、硫酸根含量测定和连接分析之前进行了纯化。我们的结果表明，日本角叉菜岩藻聚糖含有 70.13±0.22wt%岩藻糖和 15.16±1.17wt%硫酸根。还发现了其他少量的单糖，如木糖、半乳糖、甘露糖、葡萄糖、阿拉伯糖、鼠李糖和葡萄糖醛酸。连接分析表明，主链上的岩藻糖吡喃糖单元通过α-1,3-糖苷键连接，在 C-2 处分支有岩藻糖，每三个岩藻糖单元就有一个硫酸根基团在 C-4 上，即日本角叉菜岩藻聚糖的结构为[→3)-α-fuc(1→][→3)-α-fuc-4-OSO-(1→][→2)-α-fuc]。
