Department of Biomedical Sciences, Ross University School of Veterinary Medicine, P.O. Box 334, 00265 Basseterre, St. Kitts and Nevis, West Indies.
College of Animal Biotechnology, Guru Angad Dev Veterinary and Animal Science University, 141004 Ludhiana, India.
Viruses. 2021 Oct 30;13(11):2194. doi: 10.3390/v13112194.
Using a broad-range nested PCR assay targeting the DNA-dependent DNA polymerase () gene, we detected adenoviruses in 17 (20.48%) out of 83 fecal samples from small Indian mongooses () on the Caribbean island of St. Kitts. All 17 PCR amplicons were sequenced for the partial gene (300 bp, hereafter referred to as Mon sequences). Fourteen of the 17 Mon sequences shared maximum homology (98.3-99.6% and 97-98.9% nucleotide (nt) and deduced amino acid (aa) sequence identities, respectively) with that of bovine adenovirus-6 (species ). Mongoose-associated adenovirus Mon-39 was most closely related (absolute nt and deduced aa identities) to an atadenovirus from a tropical screech owl. Mon-66 shared maximum nt and deduced aa identities of 69% and 71.4% with those of atadenoviruses from a spur-thighed tortoise and a brown anole lizard, respectively. Phylogenetically, Mon-39 and Mon-66 clustered within clades that were predominated by atadenoviruses from reptiles, indicating a reptilian origin of these viruses. Only a single mongoose-associated adenovirus, Mon-34, was related to the genus . However, phylogenetically, Mon-34 formed an isolated branch, distinct from other mastadenoviruses. Since the fecal samples were collected from apparently healthy mongooses, we could not determine whether the mongoose-associated adenoviruses infected the host. On the other hand, the phylogenetic clustering patterns of the mongoose-associated atadenoviruses pointed more towards a dietary origin of these viruses. Although the present study was based on partial pol sequences (90 aa), sequence identities and phylogenetic analysis suggested that Mon-34, Mon-39, and Mon-66 might represent novel adenoviruses. To our knowledge, this is the first report on the detection and molecular characterization of adenoviruses from the mongoose.
使用针对 DNA 依赖性 DNA 聚合酶（）基因的广谱嵌套 PCR 检测方法，我们从加勒比圣基茨岛上的小印度獴（）的 83 份粪便样本中检测到 17 种腺病毒（20.48%）。所有 17 个 PCR 扩增子均进行了部分基因（300 bp，以下简称 Mon 序列）的测序。17 个 Mon 序列中有 14 个与牛腺病毒-6（种）的最大同源性（核苷酸（nt）和推导氨基酸（aa）序列同一性分别为 98.3-99.6%和 97-98.9%）。与热带 screech owl 来源的腺病毒 Mon-39 最为密切相关（绝对 nt 和推导 aa 同一性）。Mon-66 与来自 spur-thighed 龟和 brown anole lizard 的腺病毒 atadenovirus 的 nt 和推导 aa 最大同一性分别为 69%和 71.4%。系统发育分析表明，Mon-39 和 Mon-66 聚类在以爬行动物来源的 atadenovirus 为主的分支内，表明这些病毒起源于爬行动物。只有一种与獴有关的腺病毒，Mon-34，与属有关。然而，在系统发育上，Mon-34 形成了一个与其他 mastadenoviruses 不同的孤立分支。由于粪便样本是从明显健康的獴中收集的，我们无法确定这些腺病毒是否感染了宿主。另一方面，与獴相关的 atadenoviruses 的系统发育聚类模式更倾向于这些病毒的饮食来源。尽管本研究基于部分 pol 序列（90 aa），但序列同一性和系统发育分析表明，Mon-34、Mon-39 和 Mon-66 可能代表新的腺病毒。据我们所知，这是首次从獴中检测到腺病毒并对其进行分子特征描述的报告。
