College of Agronomy and Biotechnology, Southwest University, Chongqing, 400716, China.
State Key Laboratory of Cotton Biology/Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, 455000, China.
BMC Genomics. 2019 Jul 22;20(1):599. doi: 10.1186/s12864-019-5819-6.
Cotton is a leading natural fiber crop. Beyond its fiber, cottonseed is a valuable source of plant protein and oil. Due to the much higher value of cotton fiber, there is less consideration of cottonseed quality despite its potential value. Though some QTL controlling cottonseed quality have been identified, few of them that warrant further study are known. Identifying stable QTL controlling seed size, oil and protein content is necessary for improvement of cottonseed quality.
In this study, a recombinant inbred line (RIL) population was developed from a cross between upland cotton cultivars/lines Yumian 1 and M11. Specific locus amplified fragment sequencing (SLAF-seq) technology was used to construct a genetic map that covered 3353.15 cM with an average distance between consecutive markers of 0.48 cM. The seed index, together with kernel size, oil and protein content were further used to identify QTL. In total, 58 QTL associated with six traits were detected, including 13 stable QTL detected in all three environments and 11 in two environments.
A high resolution genetic map including 7033 SNP loci was constructed through specific locus amplified fragment sequencing technology. A total of 13 stable QTL associated with six cottonseed quality traits were detected. These stable QTL have the potential for fine mapping, identifying candidate genes, elaborating molecular mechanisms of cottonseed development, and application in cotton breeding programs.
棉花是主要的天然纤维作物。除了纤维，棉籽也是植物蛋白质和油的宝贵来源。由于棉花纤维的价值要高得多，尽管棉籽有潜在的价值，但对其质量的考虑就相对较少。尽管已经确定了一些控制棉籽质量的数量性状位点（QTL），但已知的值得进一步研究的 QTL 很少。鉴定控制种子大小、油和蛋白质含量的稳定 QTL 对于提高棉籽质量是必要的。
本研究以陆地棉品种/系豫棉 1 和 M11 为亲本构建重组自交系（RIL）群体，利用简化基因组测序（SLAF-seq）技术构建了一张遗传图谱，图谱全长 3353.15cM，标记间平均距离为 0.48cM。种子指数与子仁大小、油分和蛋白质含量一起用于鉴定 QTL。共检测到与 6 个性状相关的 58 个 QTL，其中在 3 个环境中均检测到 13 个稳定 QTL，在 2 个环境中检测到 11 个稳定 QTL。
利用简化基因组测序技术构建了一张包含 7033 个 SNP 标记的高分辨率遗传图谱。共检测到与 6 个棉籽品质性状相关的 13 个稳定 QTL。这些稳定的 QTL 具有精细定位、候选基因鉴定、阐明棉籽发育的分子机制以及在棉花育种计划中的应用的潜力。
