Gan L S, Skipper P L, Peng X C, Groopman J D, Chen J S, Wogan G N, Tannenbaum S R
Department of Applied Biological Sciences, Massachusetts Institute of Technology, Cambridge 02178.
Carcinogenesis. 1988 Jul;9(7):1323-5. doi: 10.1093/carcin/9.7.1323.
Aflatoxin-serum albumin adducts in the blood of 42 residents of Guangxi Province, People's Republic of China, were determined and compared with intake of aflatoxin B1 (AFB1) and excretion of aflatoxin M1 (AFM1) in urine. Blood specimens were obtained during the same period that urine was collected and that diet was sampled. Serum albumin was isolated from blood by affinity chromatography on Reactive Blue 2-Sepharose and subjected to enzymatic proteolysis using Pronase. Immunoreactive products were purified by immunoaffinity chromatography and quantified by competitive radioimmunoassay. A highly significant correlation (r = 0.60, P less than 0.00003) of adduct level with AFM1 excretion was observed. An equally highly significant correlation of adduct level with intake (r = 0.69, P less than 0.000001) was also observed. From the slope of the regression line for adduct level as a function of intake, it was calculated that 1.4-2.3% of ingested AFB1 becomes covalently bound to serum albumin, a value very similar to that observed when rats are administered AFB1.
对中华人民共和国广西省42名居民血液中的黄曲霉毒素 - 血清白蛋白加合物进行了测定，并与黄曲霉毒素B1（AFB1）的摄入量和尿中黄曲霉毒素M1（AFM1）的排泄量进行了比较。在收集尿液和采集饮食样本的同一时期采集血液样本。通过在活性蓝2 - 琼脂糖上进行亲和层析从血液中分离血清白蛋白，并用链霉蛋白酶进行酶促蛋白水解。免疫反应产物通过免疫亲和层析纯化，并通过竞争性放射免疫测定法定量。观察到加合物水平与AFM1排泄之间存在高度显著的相关性（r = 0.60，P < 0.00003）。还观察到加合物水平与摄入量之间同样高度显著的相关性（r = 0.69，P < 0.000001）。根据加合物水平作为摄入量函数的回归线斜率计算得出，摄入的AFB1中有1.4 - 2.3%与血清白蛋白共价结合，该值与给大鼠施用AFB1时观察到的值非常相似。
