Chen Gaoyun, Gong Yun Yun, Kimanya Martin E, Shirima Candida P, Routledge Michael N
a Institute for Global Food Security, School of Biological Sciences, Queen's University Belfast , Belfast , United Kingdom.
b School of Food Science and Nutrition, University of Leeds , Leeds , United Kingdom.
Biomarkers. 2018 Mar;23(2):131-136. doi: 10.1080/1354750X.2017.1285960. Epub 2017 Feb 20.
To determine levels of urinary aflatoxin M1 (AFM1) in children and correlate the concentrations with previously reported aflatoxin albumin adduct (AF-alb) levels in these children.
Matched urine and blood samples were collected from 84 Tanzanian children aged 6-14 months old. From 31 children in one village (Kigwa), samples were collected at three time points six months apart. Samples were collected from 31 and 22 children from two different regions at the second time point only. Urinary AFM1 was measured using a commercial enzyme-linked immunosorbent assay (ELISA) kit with a modified protocol to improve sensitivity. AF-alb was measured using an established ELISA method.
The relative ranking of the three villages for exposure to aflatoxin based on either AFM or AF-alb biomarker measurements was the same. In Kigwa village, both AFM1 and AF-alb levels were higher at six months post-harvest compared to baseline. However, at the next visit, the AFM1 levels dropped from a GM (interquartile range) of 71.0 (44.7, 112.6) at visit two to 49.3 (31.5, 77.3) pg/ml urine, whereas AF-alb levels increased from 47.3 (29.7, 75.2) to 52.7 (35.4, 78.3) pg/mg albumin between these two visits, reflecting the fact that AFM1 measures short-term exposure, whereas AF-alb measures longer term exposure. There was a correlation between AFB1 intake and AFM1 excretion (r= 0.442, p ≤ 0.001).
Urinary AFM1 is a good biomarker for AFB1 exposure in Tanzanian children, reflecting geographical and temporal variations in exposure to this foodborne toxin.
测定儿童尿中黄曲霉毒素M1（AFM1）水平，并将其浓度与此前报道的这些儿童的黄曲霉毒素白蛋白加合物（AF-alb）水平相关联。
收集了84名6至14个月大的坦桑尼亚儿童的配对尿液和血液样本。从一个村庄（基瓜）的31名儿童中，在相隔6个月的三个时间点采集样本。仅在第二个时间点从两个不同地区的31名和22名儿童中采集样本。使用经过改良以提高灵敏度的商业酶联免疫吸附测定（ELISA）试剂盒测量尿中AFM1。使用既定的ELISA方法测量AF-alb。
基于AFM或AF-alb生物标志物测量，三个村庄黄曲霉毒素暴露的相对排名相同。在基瓜村，收获后6个月时AFM1和AF-alb水平均高于基线。然而，在下一次访视时，AFM1水平从第二次访视时的几何均值（四分位间距）71.0（44.7，112.6）pg/ml尿液降至49.3（31.5，77.3）pg/ml，而这两次访视之间AF-alb水平从47.3（29.7，75.2）增至52.7（35.4，78.3）pg/mg白蛋白，这反映出AFM1测量短期暴露，而AF-alb测量长期暴露。黄曲霉毒素B1摄入量与AFM1排泄量之间存在相关性（r = 0.442，p≤0.001）。
尿中AFM1是坦桑尼亚儿童黄曲霉毒素B1暴露的良好生物标志物，反映了这种食源性毒素暴露的地理和时间差异。
