一种新型微电流敷料在大鼠皮肤缺损模型中的促愈合作用。
A novel microcurrent dressing for wound healing in a rat skin defect model.
机构信息
Department of Experimental Pathology, Beijing Institute of Radiation Medicine, Beijing, 100850, China.
Department of Internal Neurology, The First Affiliated Hospital/Xinxiang Medical University, Xinxiang, 453100, Henan, China.
出版信息
Mil Med Res. 2019 Jul 23;6(1):22. doi: 10.1186/s40779-019-0213-x.
BACKGROUND
The exogenous application of low-intensity electric stimulation (ES) may mimic a natural endogenous bioelectric current and accelerate the repair process of skin wounds. This study designed a novel microcurrent dressing (MCD) and evaluated its potential effects on wound healing in a rat skin defect model.
METHODS
First, wireless ES was integrated into a medical cotton cushion to fabricate the MCD, and its electrical property was examined by using a universal power meter. Then, animal experiments were conducted to evaluate the MCD's effect. Forty-five rats were randomized into control (Con) group, Vaseline gauze (VG) group and MCD group. A full-thickness round skin incision 1.5 cm in diameter was made on the back of each animal. Apart from routine disinfection, the Con rats were untreated, whereas the other two groups were treated with VG or MCD. On days 3, 7 and 14 post injury, the wound areas were observed and measured using image analysis software following photography, and the skin samples were harvested from wound tissue. Then, histopathological morphology was observed routinely by hematoxylin and eosin (HE) staining; tumor necrosis factor α (TNF-α) and interleukin (IL)-1β expression were detected by Western blotting. Vascular endothelial growth factor (VEGF) and epidermal growth factor (EGF) expression were detected with immunohistochemistry.
RESULTS
The MCD generated a sf electric potential greater than 0.95 V. Animal experiments showed that the wound-healing rate in the MCD group was significantly increased compared with the Con and VG groups (P < 0.05 or P < 0.01). Histopathological observation revealed an alleviated inflammatory response, induced vascular proliferation and accelerated epithelization in the MCD group. Moreover, samples from the MCD group expressed reduced TNF-α and IL-1β levels and increased VEGF and EGF levels compared with those of the other two groups (P < 0.05 or P < 0.01). However, no significant difference was noted between the Con and VG groups at each time point.
CONCLUSIONS
The MCD generates a stable and lasting ES and significantly promotes wound healing by reducing inflammation duration and increasing growth factors expression. Thus, MCD may act as a promising biomaterial device for skin wound healing.
背景
低强度电刺激（ES）的外源性应用可能模拟自然内源性生物电流，并加速皮肤伤口的修复过程。本研究设计了一种新型微电流敷料（MCD），并在大鼠皮肤缺损模型中评估了其对伤口愈合的潜在影响。
方法
首先，将无线 ES 集成到医用棉垫中以制造 MCD，并使用通用功率计检查其电性能。然后，进行动物实验以评估 MCD 的效果。将 45 只大鼠随机分为对照组（Con）、凡士林纱布（VG）组和 MCD 组。在每只动物的背部做一个 1.5cm 直径的全层圆形皮肤切口。除常规消毒外，Con 组大鼠不做任何处理，而其他两组大鼠分别用 VG 或 MCD 处理。在损伤后 3、7 和 14 天，通过图像分析软件观察和测量伤口面积，并从伤口组织中采集皮肤样本。然后，通过苏木精和伊红（HE）染色常规观察组织病理学形态；通过 Western blot 检测肿瘤坏死因子-α（TNF-α）和白细胞介素（IL）-1β的表达。用免疫组织化学检测血管内皮生长因子（VEGF）和表皮生长因子（EGF）的表达。
结果
MCD 产生的 sf 电势大于 0.95V。动物实验表明，与 Con 和 VG 组相比，MCD 组的伤口愈合率显著增加（P<0.05 或 P<0.01）。组织病理学观察显示，MCD 组炎症反应减轻，血管增生诱导，上皮化加速。此外，与其他两组相比，MCD 组样本中 TNF-α和 IL-1β水平降低，VEGF 和 EGF 水平升高（P<0.05 或 P<0.01）。然而，在每个时间点，Con 和 VG 组之间没有显著差异。
结论
MCD 产生稳定且持久的 ES，并通过减少炎症持续时间和增加生长因子表达来显著促进伤口愈合。因此，MCD 可能成为一种有前途的皮肤伤口愈合生物材料装置。
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