Screening and Bioprospecting of Anaerobic Consortia for Biofuel Production Enhancement from Sugarcane Bagasse.

The genera Dysgonomonas, Coprococcus, Sporomusa, Bacteroides, Sedimentibacter, Pseudomonas, Ruminococcus, and Clostridium predominate in compost residue, and vadimCA02, Anaerobaculum, Tatlockia, Caloramator, and Syntrophus prevail in soil used as inoculum in batch rectors. This mixed consortium was used as inoculum for biogas production using different concentrations of sugarcane bagasse (SCB) (from 1.58 to 4.42 g/L) and yeast extract (YE) (from 0.58 to 3.42 g/L) according to a composite central design. The maximum ethanol production (20.11 mg L) was observed using 2.0 and 3.0 g L of YE and SCB, respectively (C6). Likewise, the highest hydrogen production (0.60 mmol L) was observed using 3.0 and 4.0 g L of YE and SCB, respectively (C1). Methane was also observed, reaching the maximum production (1.44 mmol L) using 1.0 and 4.0 g L of YE and SCB, respectively (C2). The archaeal similarity between these conditions was above 90%; however, the richness and diversity were higher in the C2 (12 and 2.42, respectively) than in C1 (5 and 1.43, respectively) and C6 (11 and 2.29, respectively). Equally, the bacterial similarity between C1 and C6 was 60% while richness of 24 and 17 and diversity of 3.13 and 2.81 were observed in C1 and C6, respectively.