University of São Paulo, João Dagnone Avenue, 1100, São Carlos, SP, 13563-120, Brazil.
Federal University of São Carlos, Rod Washington Luis, Km 235, São Carlos, SP, 13565-905, Brazil.
Appl Biochem Biotechnol. 2020 Jan;190(1):232-251. doi: 10.1007/s12010-019-03074-2. Epub 2019 Jul 22.
The genera Dysgonomonas, Coprococcus, Sporomusa, Bacteroides, Sedimentibacter, Pseudomonas, Ruminococcus, and Clostridium predominate in compost residue, and vadimCA02, Anaerobaculum, Tatlockia, Caloramator, and Syntrophus prevail in soil used as inoculum in batch rectors. This mixed consortium was used as inoculum for biogas production using different concentrations of sugarcane bagasse (SCB) (from 1.58 to 4.42 g/L) and yeast extract (YE) (from 0.58 to 3.42 g/L) according to a composite central design. The maximum ethanol production (20.11 mg L) was observed using 2.0 and 3.0 g L of YE and SCB, respectively (C6). Likewise, the highest hydrogen production (0.60 mmol L) was observed using 3.0 and 4.0 g L of YE and SCB, respectively (C1). Methane was also observed, reaching the maximum production (1.44 mmol L) using 1.0 and 4.0 g L of YE and SCB, respectively (C2). The archaeal similarity between these conditions was above 90%; however, the richness and diversity were higher in the C2 (12 and 2.42, respectively) than in C1 (5 and 1.43, respectively) and C6 (11 and 2.29, respectively). Equally, the bacterial similarity between C1 and C6 was 60% while richness of 24 and 17 and diversity of 3.13 and 2.81 were observed in C1 and C6, respectively.
堆肥残渣中的优势属为 Dysgonomonas、Coprococcus、Sporomusa、Bacteroides、Sedimentibacter、Pseudomonas、Ruminococcus 和 Clostridium，而在用作批量反应器接种物的土壤中，优势属为 vadimCA02、Anaerobaculum、Tatlockia、Caloramator 和 Syntrophus。该混合菌丛被用作接种物，用于使用不同浓度的甘蔗渣 (SCB)（从 1.58 到 4.42 g/L）和酵母提取物 (YE)（从 0.58 到 3.42 g/L）进行沼气生产，根据复合中心设计进行。使用 2.0 和 3.0 g/L 的 YE 和 SCB 时，观察到最大的乙醇产量（20.11 mg/L）（C6）。同样，使用 3.0 和 4.0 g/L 的 YE 和 SCB 时，观察到最高的氢气产量（0.60 mmol/L）（C1）。也观察到了甲烷，使用 1.0 和 4.0 g/L 的 YE 和 SCB 时，甲烷的最大产量（1.44 mmol/L）（C2）。这些条件下的古菌相似度均高于 90%；然而，C2 的丰富度和多样性更高（分别为 12 和 2.42），而 C1 和 C6 则较低（分别为 5 和 1.43，以及 11 和 2.29）。同样，C1 和 C6 之间的细菌相似度为 60%，而 C1 和 C6 的丰富度分别为 24 和 17，多样性分别为 3.13 和 2.81。
