Chemistry Department, Federal University of São Carlos, Rod Washington Luis s/n, Km 235, 13565-905, São Carlos, SP, Brazil.
Laboratory of Biological Processes, Department of Hydraulics and Sanitation, Engineering School of São Carlos, University of São Paulo (EESC - USP) Campus II, 13563-120, São Carlos, SP, Brazil.
Appl Biochem Biotechnol. 2022 Jul;194(7):3145-3166. doi: 10.1007/s12010-022-03905-9. Epub 2022 Mar 29.
The production of biofuels using sugarcane bagasse (SCB) as substrate can be considered an environmentally friendly approach, due to the possibility of combining energy production with the reuse of agroindustrial wastes. This study was undertaken to explore the applicability of a new extract with the enzymes (Lac) isolated from Chaetomium cupreum for SCB pretreatment. Lac was more active at pH of 2.2 to 4 and 50 to 60 °C. Further, the individual and mutual effects of SCB concentration (6.6 to 23.4 g L), enzyme concentration (0.066 to 0.234 U L), and incubation time of the SCB with Lac (19 to 221 min) on SCB pretreatment were evaluated using a response surface methodology and central composite design. The optimized conditions were 23.4 g L SCB, 0.234 U mL laccases, and 2.44 h resulting in 547 ± 108 mg L of total sugars. This value agrees with the predicted value (455 ± 41 mg L) by the statistical model. Through the SCB pretreated with Lac fermentation, 96.1% more H and 22.5% more organic acids were observed compared to SCB without pretreatment. Therefore, laccases improve delignification, maximizing biomass fermentation for biofuel production.
利用甘蔗渣（SCB）作为底物生产生物燃料可以被认为是一种环保的方法，因为它有可能将能源生产与农业工业废物的再利用结合起来。本研究旨在探索一种新的提取方法的适用性，该方法使用从 Chaetomium cupreum 中分离出的酶（Lac）预处理 SCB。Lac 在 pH 值为 2.2 到 4 和 50 到 60°C 时活性更高。此外，使用响应面法和中心复合设计评估了 SCB 浓度（6.6 到 23.4 g L）、酶浓度（0.066 到 0.234 U L）和 Lac 与 SCB 孵育时间（19 到 221 min）对 SCB 预处理的单独和相互影响。优化条件为 23.4 g L SCB、0.234 U mL 漆酶和 2.44 h，总糖产量为 547±108 mg L。该值与统计模型预测的值（455±41 mg L）相符。通过 Lac 发酵预处理 SCB，与未经预处理的 SCB 相比，观察到 H 增加了 96.1%，有机酸增加了 22.5%。因此，漆酶可以提高脱木质素作用，使生物质发酵最大化，从而生产生物燃料。
