Department of Radiation Oncology, Cancer Hospital of Fudan University Minhang Branch, Shanghai, China.
Department of Rehabilitation, Huai'an Second People's Hospital, The Affiliated Huai'an Hospital of Xuzhou Medical University, Huai'an, China.
J Cell Physiol. 2020 Feb;235(2):993-1000. doi: 10.1002/jcp.29015. Epub 2019 Jul 22.
Cervical cancer is a tumor with the second highest morbidity and mortality in the world, and it is also the most common cancer and the eighth lethal factor among malignant tumors in Chinese female. This study aimed to identify long noncoding RNAs (lncRNAs) that related to diagnosis and prognosis in cervical cancer to improve early diagnosis and treatment. First, we extracted transcriptome profilings of cervical cancer samples from the cancer genome atlas (TCGA) database, and then extracted the lncRNAs and mRNAs expression profiles. Based on the lncRNAs expression profiles of test set, we screened lncRNAs that related to progression of cervical cancer tumors. We found six lncRNAs associated with tumor progression in cervical cancer patients, in which five lncRNAs have highly similar expression patterns but the other one has the opposite expression pattern. We found that these six lncRNAs might be related to keratinization and immunity by enrichment analysis, and two of them (AC126474 and C5orf66-AS1) were associated with prognosis in patients with cervical cancer. And these results were validated using the validation set. Overall, we identified six lncRNAs that played an important role in the development of cervical cancer, and two of them might be associated with the prognosis of cervical cancer, which provides new insight into the diagnosis and treatment of cervical cancer.
宫颈癌是全球发病率和死亡率第二高的肿瘤，也是中国女性中最常见的癌症和第八大恶性肿瘤致死因素。本研究旨在鉴定与宫颈癌诊断和预后相关的长非编码 RNA（lncRNAs），以提高早期诊断和治疗水平。首先，我们从癌症基因组图谱（TCGA）数据库中提取了宫颈癌样本的转录组谱，并提取了 lncRNAs 和 mRNAs 表达谱。基于测试集中的 lncRNAs 表达谱，我们筛选了与宫颈癌肿瘤进展相关的 lncRNAs。我们发现了六个与宫颈癌患者肿瘤进展相关的 lncRNAs，其中五个 lncRNAs 的表达模式高度相似，而另一个则相反。通过富集分析，我们发现这些六个 lncRNAs 可能与角化和免疫有关，其中两个（AC126474 和 C5orf66-AS1）与宫颈癌患者的预后相关。并且这些结果使用验证集进行了验证。总的来说，我们鉴定了六个在宫颈癌发生发展中起重要作用的 lncRNAs，其中两个可能与宫颈癌的预后相关，这为宫颈癌的诊断和治疗提供了新的见解。
