Rabasseda J, Rauret G, Galceran M T
University of Barcelona, Analytical Chemistry Department, Spain.
J Assoc Off Anal Chem. 1988 Mar-Apr;71(2):350-3.
A method for the determination of available lysine on the basis of the reactivity of the epsilon-amino group with fluorodinitrobenzene (FDNB) has been optimized. Hydrolysis is performed in closed vessels in an autoclave. Conditions for different meals were established by using a modified sequential simplex method. Hydrolysis for 4 h was sufficient for the meals studied--soybean and fish. The use of liquid chromatography to determine available lysine was studied, and optimum conditions were established for separation and quantitation of epsilon-DNB-lysine. The proposed method is faster, more accurate, and more precise than commonly used methods.
一种基于ε-氨基与氟二硝基苯（FDNB）反应性测定有效赖氨酸的方法已得到优化。水解在高压釜的密闭容器中进行。通过使用改进的序贯单纯形法确定了不同膳食的条件。对于所研究的膳食——大豆和鱼类，4小时的水解时间就足够了。研究了使用液相色谱法测定有效赖氨酸，并确定了分离和定量ε-DNB-赖氨酸的最佳条件。所提出的方法比常用方法更快、更准确、更精确。
