Song Changzheng, Yan Yifan, Rosado Abel, Zhang Zhenwen, Castellarin Simone Diego
Shaanxi Engineering Research Center for Viti-Viniculture, College of Enology, Northwest A&F University, Yangling, China.
Wine Research Centre, The University of British Columbia, Vancouver, BC, Canada.
Front Plant Sci. 2019 Jul 5;10:872. doi: 10.3389/fpls.2019.00872. eCollection 2019.
Abscisic acid (ABA) is a plant hormone that can mitigate heavy metal toxicity. Exogenous ABA and ABA mimic 1 (AM1) were applied to study the influence on Zn uptake and accumulation in L. cv. Merlot seedlings exposed to excess Zn. The seedlings were treated with either normal or excess levels of Zn in combination with applications of ABA and AM1. Excess Zn exposure resulted in decreased lateral root length, decreased photosynthesis, elevated uptake, and accumulation of Zn in roots, trunks, and stems, decreased jasmonic acid content in roots and leaves, and induced the expression of Zn transportation- and detoxification-related genes. Remarkably, in the presence of toxic amounts of Zn, the exogenous application of ABA, but not of AM1, reduced the uptake and accumulation of Zn in roots and induced higher expression of both ZIP genes and detoxification-related genes in root and leaf. These results indicate that exogenous ABA enhances the tolerance of grape seedlings to excess Zn and that AM1 is not a suitable ABA mimic compound for Zn stress alleviation in grapes.
脱落酸（ABA）是一种能够减轻重金属毒性的植物激素。施加外源ABA和ABA模拟物1（AM1）以研究其对暴露于过量锌环境下的梅洛葡萄幼苗锌吸收和积累的影响。幼苗用正常或过量水平的锌与ABA和AM1的施用相结合进行处理。过量暴露于锌导致侧根长度减少、光合作用降低、锌在根、树干和茎中的吸收和积累增加、根和叶中茉莉酸含量降低，并诱导锌运输和解毒相关基因的表达。值得注意的是，在存在毒性量锌的情况下，外源施用ABA而非AM1，降低了根中锌的吸收和积累，并诱导根和叶中ZIP基因和解毒相关基因的更高表达。这些结果表明，外源ABA增强了葡萄幼苗对过量锌的耐受性，并且AM1不是用于缓解葡萄锌胁迫的合适ABA模拟化合物。
