microRNA-211 通过靶向 EZRIN 调节人骨肉瘤细胞的增殖、凋亡和迁移/侵袭。
microRNA-211 regulates cell proliferation, apoptosis and migration/invasion in human osteosarcoma via targeting EZRIN.
机构信息
1Central laboratory, ZhongShan Hospital XiaMen University, Xiamen, 361004 China.
2Fujian Provincial Key Laboratory of Chronic Liver Disease and Hepatocellular Carcinoma (Xiamen University Affiliated ZhongShan Hospital), Xiamen, 361004 China.
出版信息
Cell Mol Biol Lett. 2019 Jul 9;24:48. doi: 10.1186/s11658-019-0173-x. eCollection 2019.
BACKGROUND
In recent years, microRNA-211 (miR211) has been considered as a tumor suppressor in multiple malignancies. However, the function of miR211 in human osteosarcoma has not been explored intensively so far. In this study, the relationship between miR211 and EZRIN was analyzed in human osteosarcoma.
METHODS
The expression levels of miR211 and EZRIN were measured in both human osteosarcoma cells and tissues. The direct regulatory relationship between miR211 and EZRIN was evaluated using dual-luciferase assay. The effect of miR211 and EZRIN overexpression on cell proliferation, migration/invasion, and apoptosis was detected.
RESULTS
The expression of miR211 was obviously lower in osteosarcoma tissues than paracancerous tissues. EZRIN was identified as the direct target of miR211, and up-regulation of miR211 increased the percentage of cell apoptosis, and suppressed cell proliferation as well as cell migration/invasion via directly regulating EZRIN.
CONCLUSIONS
Our study indicated that miR211 has an important role in the development and progress of osteosarcoma, and it might become a novel target in the diagnosis and treatment of human osteosarcoma.
背景
近年来，microRNA-211（miR211）被认为是多种恶性肿瘤的肿瘤抑制因子。然而，miR211 在人骨肉瘤中的功能迄今尚未得到深入探讨。在本研究中，分析了 miR211 与 EZRIN 在人骨肉瘤中的关系。
方法
检测人骨肉瘤细胞和组织中 miR211 和 EZRIN 的表达水平。利用双荧光素酶报告基因实验评估 miR211 和 EZRIN 之间的直接调控关系。检测 miR211 和 EZRIN 过表达对细胞增殖、迁移/侵袭和凋亡的影响。
结果
miR211 在骨肉瘤组织中的表达明显低于癌旁组织。EZRIN 被鉴定为 miR211 的直接靶基因，上调 miR211 通过直接调节 EZRIN 增加细胞凋亡的百分比，并抑制细胞增殖以及细胞迁移/侵袭。
结论
本研究表明，miR211 在骨肉瘤的发生发展中具有重要作用，可能成为人类骨肉瘤诊断和治疗的新靶点。
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