Chen Shaomu, Feng Yu, Zhang Biao, Chen Xiaochun, Wei Wenxiang, Ma Haitao
Department of Cell Biology, School of Medicine, Soochow University, Suzhou, Jiangsu, China.
Department of Thoracic Surgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China.
J Cancer. 2019 Jun 9;10(16):3698-3705. doi: 10.7150/jca.32680. eCollection 2019.
RMP is a RNA polymerase II Subunit RPB-5 associated protein shown to act as an oncogene in several cancer. However, the mechanism of the involvement of RMP in esophageal cancer (EC) remains unclear. We analyzed RMP expression in EC cell lines and EC tissues. The connection between RMP and clinical pathological features of EC was also elucidated. To investigate the role of RMP in EC, We performed CCK-8 assay to evaluate cell proliferation, and Annexin V/PI double-staining to evaluate cell apoptosis. Effect of RMP on tumor progression in nude mouse models was assessed by measurement of volume and weight of tumors. Expression of RMP, CEA and CA199 in vivo were measured by Inmunohistochemical staining. First of all, our study showed that RMP was highly expressed in EC cell lines (compared with normal cells) and tumor tissues (compare with corresponding normal tissues). Then, we found that RMP was bound up with the status of nodal and T stage which indicating that RMP may be related to the growth and malignant degree of EC. Moreover upregulation of RMP could contribute to tumor growth in vitro and vivo. In addition, the results also showed that overexpression of RMP could significantly reduce the susceptibility to radiotherapy. Taken together, all these further suggested that RMP would play a chance-promoting in EC which may provide us a powerful goal for gene targeting treatment of esophageal cancer.
RMP是一种与RNA聚合酶II亚基RPB - 5相关的蛋白质，已证实在多种癌症中作为癌基因发挥作用。然而，RMP在食管癌（EC）中的作用机制仍不清楚。我们分析了RMP在EC细胞系和EC组织中的表达情况。还阐明了RMP与EC临床病理特征之间的联系。为了研究RMP在EC中的作用，我们进行了CCK - 8试验以评估细胞增殖，并进行Annexin V/PI双染以评估细胞凋亡。通过测量肿瘤体积和重量评估RMP对裸鼠模型肿瘤进展的影响。通过免疫组织化学染色测量体内RMP、CEA和CA199的表达。首先，我们的研究表明RMP在EC细胞系（与正常细胞相比）和肿瘤组织（与相应正常组织相比）中高表达。然后，我们发现RMP与淋巴结和T分期状态相关，这表明RMP可能与EC的生长和恶性程度有关。此外，RMP的上调可促进体内外肿瘤生长。另外，结果还表明RMP的过表达可显著降低放疗敏感性。综上所述，所有这些进一步表明RMP在EC中起促癌作用，这可能为我们提供食管癌基因靶向治疗的有力靶点。
