Department of Radiation Oncology, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui, 230031, China.
Department of Cancer Epigenetics Program, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui, 230031, China.
Biomed Pharmacother. 2019 Oct;118:109358. doi: 10.1016/j.biopha.2019.109358. Epub 2019 Aug 29.
Esophageal cancer (EC) is one of the most difficult malignancies to cure. The radioresistance remains a major obstacle for effective treatment of EC.
Esophageal cancer radioresistant cell lines were screened by the cumulative dose of radiation assays. qRT-PCR and Western blotting assays were used to detect the expression of LMXIB in EC. The survival fraction experiments were performed to test the effects of LMXIB in the radioresistance of EC. Following, the ionizing radiation and clonogenic, cell proliferation, wound healing and Flow cytometry apoptosis assays were used to characterize the properties of these cell lines.
We found that the LMX1B gene, encoding a LIM-homeodomain transcription factor, is expressed in a higher level in the radio-resistant EC cell lines. The following radiation assays revealed that LMX1B promotes the radioresistance of EC cells. Moreover, LMX1B plays roles in the proliferation, cell migration and apoptosis of EC cells.
All these results indicated that LMX1B is a key regulator involved in the radioresistance of EC, which might be used as a biomarker to guide the clinical therapy of EC.
食管癌（EC）是最难治愈的恶性肿瘤之一。放射抵抗仍然是有效治疗 EC 的主要障碍。
通过累积剂量照射试验筛选食管癌细胞放射抵抗细胞系。qRT-PCR 和 Western blot 检测 EC 中 LMXIB 的表达。生存分数实验用于检测 LMXIB 对 EC 放射抵抗的影响。随后，采用电离辐射和集落形成、细胞增殖、伤口愈合和流式细胞术凋亡实验来表征这些细胞系的特性。
我们发现，编码 LIM 同源域转录因子的 LMX1B 基因在放射抵抗的 EC 细胞系中表达水平更高。以下的放射实验表明 LMX1B 促进了 EC 细胞的放射抵抗。此外，LMX1B 还在 EC 细胞的增殖、细胞迁移和凋亡中发挥作用。
这些结果表明，LMX1B 是参与 EC 放射抵抗的关键调节因子，可作为指导 EC 临床治疗的生物标志物。
