Kepekci Ahmet Hamdi, Ergul Zehragul, Gultekin Alper, Karaoz Erdal
Meltem Hospital, Department of Otolaryngology Istanbul, Turkey.
Istanbul Yeni Yuzyil University, Vocational Health High School, Department of Audiometry Istanbul, Turkey.
Int J Physiol Pathophysiol Pharmacol. 2019 Jun 15;11(3):76-82. eCollection 2019.
Osteoporosis is a disease characterized by an increase in bone fragility as a result of decreased bone mass and weakening of the bone structure. There are studies on the relationship between osteoporosis and hearing and balance system. The goal of this study was to compare the proliferation and osteogenesis induction properties of mesenchymal stem cells derived from healthy and osteoporotic individuals to better understand the healing properties of Korean red ginseng (KRG) and Panax ginseng-Avena Sativa-Tribulus Terrestris mixture (PAT).
Osteoporotic and healthy MSCs were isolated successfully in culture conditions. The proliferation levels of cells treated with different doses of KRG and PAT were compared by Water-Soluble Tetrazolium-1 (WST-1) assay. Alkaline phosphatase (ALP) assay was performed by selecting the most effective KRG dose in proliferation.
Morphology of isolated cells and the expression of cell surface antigens have been detected as similar. The WST-1 assay showed that KRG was effective on the proliferation of osteoporotic cells. The levels of ALP in osteoporotic cells treated with KRG is increased depending on the differentiation day compared to healthy cells.
KRG triggered an increase in intracellular ALP levels of osteoporotic MSCs. It suggests that KRG on osteoporotic cells is influential in stimulating osteogenesis and may be useful in osteoporotic patients.
骨质疏松症是一种由于骨量减少和骨结构减弱导致骨脆性增加的疾病。关于骨质疏松症与听力和平衡系统之间的关系已有相关研究。本研究的目的是比较健康个体和骨质疏松个体来源的间充质干细胞的增殖和成骨诱导特性，以更好地了解韩国红参（KRG）和人参 - 燕麦 - 刺蒺藜混合物（PAT）的愈合特性。
在培养条件下成功分离出骨质疏松和健康的间充质干细胞。通过水溶性四氮唑盐 -1（WST -1）测定法比较用不同剂量的KRG和PAT处理的细胞的增殖水平。通过在增殖中选择最有效的KRG剂量进行碱性磷酸酶（ALP）测定。
已检测到分离细胞的形态和细胞表面抗原的表达相似。WST -1测定表明KRG对骨质疏松细胞的增殖有效。与健康细胞相比，用KRG处理的骨质疏松细胞中ALP的水平根据分化天数增加。
KRG引发了骨质疏松间充质干细胞细胞内ALP水平的增加。这表明KRG对骨质疏松细胞在刺激成骨方面有影响，可能对骨质疏松患者有用。
