3300 del A-1061 Ter移码突变和丙酸钙对氧化应激及乳腺癌发生的影响
Effects of 3300 del A-1061 Ter frameshift mutation and calcium propionate on oxidative stress and breast carcinogenesis.
作者信息
Pongsavee Malinee
机构信息
Department of Medical Technology, Faculty of Allied Health Sciences, Thammasat University, Rangsit Campus Patumthani 12121, Thailand.
出版信息
Int J Mol Epidemiol Genet. 2019 Jun 15;10(3):47-52. eCollection 2019.
gene mutation increases risk of hereditary breast/ovarian carcinogenesis. Calcium propionate is the food preservative. Superoxide dismutase enzyme protects oxidative stress in human. This report studied about the effects of 3300 del A-1061 Ter mutation at exon 11 and calcium propionate toxicity on parameters of oxidative stress induction and cause of breast cancer. The effects of 3300 del A-1061 Ter frameshift mutation on oxidative stress protection were studied by MTT dye reduction assay and superoxide dismutase activity assay. Calcium propionate and 3300 del A-1061 Ter frameshift mutation effects on superoxide dismutase activity were studied by superoxide dismutase activity assay on breast cancer cell line. The results showed that this mutation caused oxidative stress through superoxide dismutase activity inhibition ( < 0.05) and calcium propionate effected on superoxide dismutase activity in breast cancer cells ( < 0.05). 3300 del A-1061 Ter mutation and the chemical toxicity effect on oxidative stress and may lead to breast carcinogenesis.
基因突变会增加遗传性乳腺癌/卵巢癌发生的风险。丙酸钙是一种食品防腐剂。超氧化物歧化酶可保护人体免受氧化应激。本报告研究了第11外显子上的3300 del A-1061 Ter突变以及丙酸钙毒性对氧化应激诱导参数和乳腺癌病因的影响。通过MTT比色法和超氧化物歧化酶活性测定研究了3300 del A-1061 Ter移码突变对氧化应激保护的影响。通过对乳腺癌细胞系进行超氧化物歧化酶活性测定，研究了丙酸钙和3300 del A-1061 Ter移码突变对超氧化物歧化酶活性的影响。结果表明，该突变通过抑制超氧化物歧化酶活性导致氧化应激（P<0.05），且丙酸钙对乳腺癌细胞中的超氧化物歧化酶活性有影响（P<0.05）。3300 del A-1061 Ter突变和化学毒性对氧化应激有影响，可能导致乳腺癌发生。
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