Gen-Probe商业试剂盒与培养技术在肺炎支原体感染诊断中的比较。
Comparison of Gen-Probe commercial kit and culture technique for the diagnosis of Mycoplasma pneumoniae infection.
作者信息
Dular R, Kajioka R, Kasatiya S
机构信息
Public Health Laboratory, Ontario Ministry of Health, Ottawa, Canada.
出版信息
J Clin Microbiol. 1988 May;26(5):1068-9. doi: 10.1128/jcm.26.5.1068-1069.1988.
Abstract
The Gen-Probe rapid diagnostic system was compared with a culture method for the detection of Mycoplasma pneumoniae in clinical specimens. Of 116 clinical specimens, 103 (88.8%) yielded identical results. The relative sensitivity and specificity of the probe were both 89%. Rapid turnaround time and its sensitivity and specificity indicate that the probe test is a practical method for the rapid diagnosis of M. pneumoniae infections.
摘要
将Gen - Probe快速诊断系统与一种用于检测临床标本中肺炎支原体的培养方法进行了比较。在116份临床标本中，103份（88.8%）得出了相同的结果。该探针的相对灵敏度和特异性均为89%。快速的周转时间及其灵敏度和特异性表明，该探针检测是一种快速诊断肺炎支原体感染的实用方法。
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本文引用的文献
1
Atypical pneumonias.
Med Clin North Am. 1980 May;64(3):507-27. doi: 10.1016/s0025-7125(16)31607-8.
2
Isolation of Mycoplasma pneumoniae from respiratory tract specimens in Ontario.安大略省呼吸道标本中肺炎支原体的分离
CMAJ. 1987 Jul 1;137(1):48-50.
3
Mycoplasma pneumoniae infections in a rural setting in Canada.加拿大农村地区的肺炎支原体感染
CMAJ. 1987 Jun 15;136(12):1271-3.
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