Singh Gurtej, Cordero John, Wiles Brody, Tembelis Miltiadis N, Liang Kai-Li, Rafailovich Miriam, Simon Marcia, Khan Sami U, Bui Duc T, Dagum Alexander B
Division of Plastic and Reconstructive Surgery, Department of Surgery, Stony Brook Medicine, Stony Brook, N.Y.
Division of Plastic and Reconstructive Surgery, Department of Surgery, Stony Brook University School of Medicine, Stony Brook, N.Y.
Plast Reconstr Surg Glob Open. 2019 May 23;7(5):e2264. doi: 10.1097/GOX.0000000000002264. eCollection 2019 May.
The use of vascular grafts is continuing to rise due to the increasing prevalence of coronary artery bypass grafting and microvascular flap-based tissue reconstructions. The current options of using native vessels (saphenous vein) or the synthetic grafts (Dacron) have been unable to manage current needs. In this study, we employed an original tissue engineering approach to develop a multi-layered vascular graft that has the potential to address some of the limitations of the existing grafts.
Biomaterials, gelatin and fibrin, were used to develop a two-layered vascular graft. The graft was seeded with endothelial cells and imaged using confocal microscopy. The graft's architecture and its mechanical properties were also characterized using histology, Scanning Electron Microscopy and rheological studies.
Our methodology resulted in the development of a vascular graft with precise spatial localization of the two layers. The endothelial cells fully covered the lumen of the developed vascular graft, thus providing a non-thrombogenic surface. The elastic modulus of the biomaterials employed in this graft was found to be 5.186 KPa, paralleling that of internal mammary artery. The burst pressure of this graft was also measured and was found close to that of the saphenous vein (~2000 mm Hg).
We were successfully able to employ a unique method to synthesize a multi-layered vascularized graft having adequate biological and mechanical properties. Studies are ongoing involving implantation of this developed vascular graft in the rat femoral artery and characterization of parameters such as vascular remodeling and patency.
由于冠状动脉旁路移植术和基于微血管皮瓣的组织重建的患病率不断上升，血管移植物的使用持续增加。目前使用自体血管（大隐静脉）或合成移植物（涤纶）的选择已无法满足当前需求。在本研究中，我们采用了一种原创的组织工程方法来开发一种多层血管移植物，该移植物有可能解决现有移植物的一些局限性。
使用生物材料明胶和纤维蛋白来开发双层血管移植物。将内皮细胞接种到移植物上，并使用共聚焦显微镜成像。还使用组织学、扫描电子显微镜和流变学研究对移植物的结构及其力学性能进行了表征。
我们的方法导致开发出一种两层具有精确空间定位的血管移植物。内皮细胞完全覆盖了所开发血管移植物的管腔，从而提供了一个抗血栓形成的表面。发现该移植物中使用的生物材料的弹性模量为5.186千帕，与胸廓内动脉的弹性模量相当。还测量了该移植物的破裂压力，发现其接近大隐静脉的破裂压力（约2000毫米汞柱）。
我们成功地采用了一种独特的方法来合成一种具有足够生物学和力学性能的多层血管化移植物。正在进行的研究包括将这种开发的血管移植物植入大鼠股动脉，并对血管重塑和通畅性等参数进行表征。
