Agrawal Surendra S, Yallatikar Tapasya, Gurjar Pravina
Department of Quality Assurance, Shobhaben Pratapbhai Patel School of Pharmacy and Technology Management, SVKM'S NMIMS, Mumbai, India.
Department of Pharmaceutics, Sharadchandra Pawar College of Pharmacy, Pune, Maharashtra, India.
J Adv Pharm Technol Res. 2019 Jul-Sep;10(3):126-131. doi: 10.4103/japtr.JAPTR_382_18.
A simple, specific, accurate, precise, and robust reverse-phase high-performance liquid chromatographic method has been developed and validated for the estimation of allyl isothiocyanate (AITC) in phytosomes of black mustard extract (). The method was validated with respect to specificity, linearity, accuracy, precision, and robustness. The linearity was achieved over a range of 10-18 μg/mL and regression coefficient was obtained 0.9961. Accuracy of chromatographic method was evaluated by standard addition method; percentage recovery attained was 97.07 ± 0.008-103.66 ± 0.013. Relative standard deviation for intraday and interday precision was 0.02% and 0.22%, respectively. The limit of detection and limit of quantification of the AITC were found to be 0.0043 μg/mL and 0.01324 μg/mL, respectively. This result shows that the method was well validated. In the present study, the AITC content was found 0.0009% ± 0.014% in black mustard. This study reveals that the proposed high-performance thin-layer chromatography method is accurate, fast, and cost-effective for the routine estimation of AITC in the phytosome formulation.
已开发并验证了一种简单、特异、准确、精密且稳健的反相高效液相色谱法，用于测定黑芥子提取物磷脂体中异硫氰酸烯丙酯（AITC）的含量。该方法在特异性、线性、准确性、精密度和稳健性方面进行了验证。在10 - 18μg/mL范围内实现了线性，回归系数为0.9961。采用标准加入法评估色谱方法的准确性；回收率为97.07±0.008 - 103.66±0.013。日内和日间精密度的相对标准偏差分别为0.02%和0.22%。AITC的检测限和定量限分别为0.0043μg/mL和0.01324μg/mL。结果表明该方法得到了良好的验证。在本研究中，黑芥子中AITC含量为0.0009%±0.014%。本研究表明，所提出的高效薄层色谱法对于磷脂体制剂中AITC的常规测定准确、快速且具有成本效益。
