Somwong Pathom, Theanphong Orawan
Department of Pharmaceutical Chemistry, College of Pharmacy, Rangsit University, Pathum Thani, Thailand.
Department of Pharmacognosy, College of Pharmacy, Rangsit University, Pathum Thani, Thailand.
J Adv Pharm Technol Res. 2021 Apr-Jun;12(2):147-151. doi: 10.4103/japtr.JAPTR_13_21. Epub 2021 Apr 27.
, , and have traditionally been used as herbal remedies for pain relief in Thailand. The ethanolic extracts of these plants obtained by Soxhlet extraction were analyzed by the developed high-performance liquid chromatography-diode-array detection method. Lupeol, the anti-inflammatory triterpene, was selected as a chemical marker for this investigation. All extracts together with that compound were further evaluated for their potential on anti-inflammatory activity using 5-lipoxygenase inhibition assay. Lupeol in each extract was quantified and expressed in the range of 21.44 ± 0.89-40.72 ± 0.40 mg per 100 g of crude drug and the enzyme inhibitory activity of all tested extracts presented as half-maximal inhibitory concentration values ranged between 63.71 ± 2.09 and 91.09 ± 1.40 μg/mL. This study shows that the developed analytical method is effective for analyzing triterpene lupeol in these plants and also reveals the relationship between a lupeol content and the anti-inflammatory effect.
在泰国，[植物名称1]、[植物名称2]和[植物名称3]传统上一直被用作缓解疼痛的草药。通过索氏提取法获得的这些植物的乙醇提取物，采用所开发的高效液相色谱 - 二极管阵列检测法进行分析。抗炎三萜类化合物羽扇豆醇被选为本次研究的化学标志物。使用5 - 脂氧合酶抑制试验，对所有提取物以及该化合物的抗炎活性潜力进行了进一步评估。对每种提取物中的羽扇豆醇进行定量，其含量以每100克粗药中21.44±0.89 - 40.72±0.40毫克的范围表示，所有测试提取物的酶抑制活性以半数最大抑制浓度值表示，范围在63.71±2.09至91.09±1.40微克/毫升之间。本研究表明，所开发的分析方法对于分析这些植物中的三萜类化合物羽扇豆醇是有效的，并且还揭示了羽扇豆醇含量与抗炎作用之间的关系。
