Jangle R D, Thorat B N
Department of Chemical Engineering, Advanced Drying Laboratory, Institute of Chemical Technology (Formerly UDCT), N. P. Road, Matunga (E), Mumbai-400 019, India.
Indian J Pharm Sci. 2013 Jan;75(1):60-6. doi: 10.4103/0250-474X.113555.
A simple, sensitive, precise and specific method for the determination of curcuminoids and curcuminoid-loaded liposome formulation was developed using reverse-phase high-performance liquid chromatography method. The analysis was performed isocratically on Zorbax Eclipse XDB-C18 column (150×4 mm, 5 μm), analytical column using UV detector and mobile phase consisting of 0.1% orthophosphoric acid and acetonitrile. The proposed method for curcuminoids was validated for linearity in the range from 50 to 300 ΅g/ml with correlation coefficient above 0.997. Intraday and interday precision studies showed the relative standard deviation less than 2%. The limit of detection and limit of quantitation values were 2.5 and 8.25 ΅g/ml, respectively. Forced degradation study for curcuminoids and liposomal curcuminoids sample was carried out and observed that proposed method was also suitable for finding degradation products in the sample. Proposed method was successfully applied to estimate curcuminoids content without any interference of other excipients from liposomal formulation. Therefore, the method developed is well suited for curcuminoids and its liposome estimation.
采用反相高效液相色谱法建立了一种简单、灵敏、准确且特异的测定姜黄素类化合物及载姜黄素脂质体制剂的方法。分析在Zorbax Eclipse XDB-C18柱（150×4 mm，5 μm）上进行等度洗脱，使用紫外检测器作为分析柱，流动相由0.1%的正磷酸和乙腈组成。所提出的姜黄素类化合物测定方法在50至300 μg/ml范围内进行线性验证，相关系数高于0.997。日内和日间精密度研究表明相对标准偏差小于2%。检测限和定量限分别为2.5和8.25 μg/ml。对姜黄素类化合物和脂质体姜黄素类化合物样品进行了强制降解研究，发现所提出的方法也适用于检测样品中的降解产物。所提出的方法成功应用于估计姜黄素类化合物含量，不受脂质体制剂中其他辅料的干扰。因此，所开发的方法非常适合姜黄素类化合物及其脂质体的测定。
