Fatemi Nayeri Mahdieh, Talaei Ali, Tavakkol Afshari Jalil, Nikpoor Amin Reza, Talaei Andisheh, Ganjali Rashin
Psychiatry and Behavioral Sciences Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
Immunology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
Rep Biochem Mol Biol. 2019 Apr;8(1):1-8.
The pathophysiology of bipolar 1 disorder (B1D), a major psychiatric disorder with inflammatory origins and structural changes in the brain, is of great interest to researchers. Pro-inflammatory biomarkers and specific gene expression play pivotal roles in B1D development, and IFN-γ has emerged as an important inflammatory marker. The aim of this research was to determine whether the INF-γ +874 T/A polymorphism is associated with B1D susceptibility in an ethnic Iranian population.
The IFN-γ +874 T/A (rs2430561) gene polymorphism was studied in 106 B1D patients and 109 control subjects using sequence specific primers (SSPs) and amplification refractory mutation system-polymerase chain reaction (ARMS-PCR).
Significant statistical differences in IFN-γ +874 T/A polymorphism genotype distribution were found between the patients and control subjects (P = 0.0006). Decreased risk of B1D was detected in the codominant model (T/T vs T/A and A/A, OR = 0.19, 95% CI = 0.07-0.49 for T/A, OR = 0.38, 95% CI = 0.12-1.24 for A/A, P value=0.0006), and in the dominant model (T/T vs T/A-A/A, OR = 0.21, 95% CI = 0.08-0.54, P = 0.0005). However, no significant difference in the IFN-γ polymorphism allele distribution was found between the two groups (P = 0.25).
The IFN-γ +874 T/A polymorphism may have a significant role in BID development.
双相I型障碍（B1D）是一种具有炎症起源和大脑结构变化的主要精神疾病，其病理生理学引起了研究人员的极大兴趣。促炎生物标志物和特定基因表达在B1D的发展中起关键作用，而干扰素-γ（IFN-γ）已成为一种重要的炎症标志物。本研究的目的是确定IFN-γ +874 T/A多态性是否与伊朗人群中B1D的易感性相关。
采用序列特异性引物（SSP）和扩增阻滞突变系统-聚合酶链反应（ARMS-PCR）对106例B1D患者和109例对照者的IFN-γ +874 T/A（rs2430561）基因多态性进行研究。
患者与对照者之间IFN-γ +874 T/A多态性基因型分布存在显著统计学差异（P = 0.0006）。在共显性模型中检测到B1D风险降低（T/T与T/A和A/A相比，T/A的OR = 0.19，95% CI = 0.07 - 0.49，A/A的OR = 0.38，95% CI = 0.12 - 1.24，P值 = 0.0006），在显性模型中也降低（T/T与T/A - A/A相比，OR = 0.21，95% CI = 0.08 - 0.54，P = 0.0005）。然而，两组之间IFN-γ多态性等位基因分布无显著差异（P = 0.25）。
IFN-γ +874 T/A多态性可能在BID的发展中起重要作用。
