Tallei Trina Ekawati, Pelealu Johanis Jullian
Department of Biology, Faculty of Mathematics and Natural Sciences, Sam Ratulangi University, Indonesia.
Pharmacy Study Program, Faculty of Mathematics and Natural Sciences, Sam Ratulangi University, Indonesia.
Data Brief. 2019 Jun 25;25:104190. doi: 10.1016/j.dib.2019.104190. eCollection 2019 Aug.
This article describes the data on metagenomic profile of the bacterial community and diversity in the brine of fermented romaine lettuce in two experimental brine salinity (5 and 10%) obtained by Illumina MiSeq sequencing of V3-V4 region of 16S rRNA gene. A total of 98,660 reads (10%) and 95,968 (5%) consisted 38 and 47 consensus lineages (OTUs), respectively. Predominating phyla in 10% were 55.89% Proteobacteria and 41.97% Firmicutes, while predominating phyla in 5% brine were 63.47% Proteobacteria and 34.60% Firmicutes. The predominating lower taxa in 10% brine were Haererehalobacter salaria (54.19%) and Bacillaceae (33.2%), while in 5% brine were Enterobacteriaceae (46.67%) and Bacillus (21.53%). Leuconostoc (6.97%) and Lactococcus (3.97%) only appeared in 5% brine. The data presented here is the first metagenomic profile of romaine lettuce fermentation in different brine concentration and will serve as a baseline to understand the shifting of bacterial diversity associated with the change in brine concentration.
本文描述了通过对16S rRNA基因V3 - V4区域进行Illumina MiSeq测序，在两种实验盐水盐度（5%和10%）下发酵长叶生菜盐卤中细菌群落的宏基因组概况和多样性数据。分别有98,660条读数（10%）和95,968条读数（5%），共组成38个和47个共有谱系（操作分类单元，OTUs）。10%盐度下的优势菌门为变形菌门（55.89%）和厚壁菌门（41.97%），而5%盐卤中的优势菌门为变形菌门（63.47%）和厚壁菌门（34.60%）。10%盐卤中的主要低分类单元为盐卤嗜盐杆菌（54.19%）和芽孢杆菌科（33.2%），而在5%盐卤中为肠杆菌科（46.67%）和芽孢杆菌属（21.53%）。明串珠菌属（6.97%）和乳球菌属（3.97%）仅出现在5%的盐卤中。这里呈现的数据是不同盐卤浓度下长叶生菜发酵的首个宏基因组概况，将作为了解与盐卤浓度变化相关的细菌多样性变化的基线。
