Lombogia Christian Apolinaris, Tulung Max, Posangi Jimmy, Tallei Trina Ekawati
Entomology Study Program, Postgraduate Program, Sam Ratulangi University, Manado, North Sulawesi, Indonesia.
Nursing Study Program, Faculty of Nursing, De La Salle Catholic University, Manado, North Sulawesi, Indonesia.
Int J Microbiol. 2020 Jul 30;2020:6906921. doi: 10.1155/2020/6906921. eCollection 2020.
Understanding the honeybee gut bacteria is an essential aspect as honeybees are the primary pollinators of many crops. In this study, the honeybee-associated gut bacteria were investigated by targeting the V3-V4 region of 16S rRNA genes using the Illumina MiSeq. The adult worker was captured in an urban area in a dense settlement. In total, 83,018 reads were obtained, revealing six phyla from 749 bacterial operational taxonomic units (OTUs). The gut was dominated by Proteobacteria (58% of the total reads, including Enterobacteriaceae 28.2%, 6.43%, and 4.90%), Firmicutes (29% of the total reads, including 13.45%, spp. 8.19%, and spp. 4.47%), and Actinobacteria (8% of the total reads, including spp. 7.96%). Many of these bacteria belong to the group of lactic acid bacteria (LAB), which was claimed to be composed of beneficial bacteria involved in maintaining a healthy host. The honeybee was identified as based on an identity BLAST search of its COI region. This study is the first report on the gut microbial community structure and composition of from Indonesia.
了解蜜蜂肠道细菌是一个重要方面，因为蜜蜂是许多作物的主要传粉者。在本研究中，使用Illumina MiSeq靶向16S rRNA基因的V3 - V4区域，对与蜜蜂相关的肠道细菌进行了调查。成年工蜂在一个密集居住区的市区被捕获。总共获得了83,018条读数，从749个细菌操作分类单元（OTU）中揭示了六个门。肠道中以变形菌门（占总读数的58%，包括肠杆菌科28.2%、 6.43%和4.90%）、厚壁菌门（占总读数的29%，包括 13.45%、 属8.19%和 属4.47%）和放线菌门（占总读数的8%，包括 属7.96%）为主。这些细菌中的许多属于乳酸菌（LAB）组，据称该组由参与维持宿主健康的有益细菌组成。根据其COI区域的同源性BLAST搜索，该蜜蜂被鉴定为 。本研究是关于来自印度尼西亚的 的肠道微生物群落结构和组成的首次报告。
