Departament of Nutrition and Health. Federal University of Viçosa, Av. Purdue, s/n, Campus Universitário, Viçosa, MG 36.570-900, Brazil.
Food Funct. 2019 Aug 1;10(8):4861-4867. doi: 10.1039/c9fo00651f. Epub 2019 Jul 23.
This study investigates the acute anti-inflammatory activity of Mangifera indica L. leaf extract and mangiferin in the liver of rats fed a cafeteria diet. This study was a randomized longitudinal experimental study. The animals were divided into three groups - Control: cafeteria diet (CD); Extract: CD + leaf extract (250 mg kg); and Mangiferin: CD + mangiferin (40 mg kg). Body weight and food intake were measured every week. On day eight, mRNA and protein expression of inflammatory markers were evaluated in the liver. Also, liver weight, SOD activity and malondialdehyde concentration were measured. Treatment for only eight days with mango leaf extract and mangiferin increased SOD activity. Mangiferin intake increased the mRNA expression of PPAR-α and HSP72. The leaf extract treatment enhanced PPAR-α mRNA expression. Mangiferin and leaf extract consumption caused a lower concentration of NFκB (p65) in nuclear extracts, and greater IL-10 mRNA and protein levels. This study highlights the potential of acute treatment with mango leaf extract and mangiferin to prevent liver inflammation caused by fat-rich diets. These results indicate a new use for a product that has low cost, is found in great amounts, and is not routinely used.
本研究旨在探究芒果叶提取物和芒果苷对 cafeteria 饮食喂养大鼠肝脏的急性抗炎活性。本研究为随机纵向实验研究。动物被分为三组：对照组（Cafeteria diet，CD）；提取物组（CD+250mg/kg 叶提取物）；和芒果苷组（CD+40mg/kg 芒果苷）。每周测量体重和食物摄入量。第八天，评估肝脏中炎症标志物的 mRNA 和蛋白表达。同时，测量肝重、SOD 活性和 MDA 浓度。仅用芒果叶提取物和芒果苷处理 8 天即可提高 SOD 活性。芒果苷摄入增加了 PPAR-α 和 HSP72 的 mRNA 表达。提取物处理增强了 PPAR-α mRNA 表达。芒果苷和叶提取物的消耗导致核提取物中 NFκB（p65）浓度降低，IL-10 的 mRNA 和蛋白水平升高。本研究强调了富含脂肪的饮食引起肝脏炎症时，急性使用芒果叶提取物和芒果苷的潜力。这些结果为一种低成本、大量存在且未常规使用的产品提供了新的用途。
