Qureshi Azam, Murphy Emily, Milando Rose, Rengifo-Pardo Monica, Clayton Courtney, Friedman Adam
J Drugs Dermatol. 2019 Jul 1;18(7):667-673.
Background: Collagen-based products have been implemented in wound healing due to collagen’s hemostatic properties, low antigenicity, and poor culture ability. Objective: To compare the rate and quality of full-thickness wound healing for topical collagen powder and primary closure. Methods: Eight volunteers received one 4 mm punch biopsy on each thigh. One wound was managed with primary closure while the other received daily collagen powder. Wounds were biopsied at four weeks for histopathological analysis. Subjects rated itch, pain, and treatment preferences at weeks 1, 2, 4, 6, and 12. Results: Six out of eight collagen-treated wounds were completely healed 4 weeks after initial wounding. Histologic analysis of the wounds revealed epidermal re-epithelization in both groups. More organized granulation tissue was noted in collagen-treated wounds and confirmed using Masson trichrome and CD31 staining for collagen and neoangiogenesis, respectively. Subjects reported similar itch and pain metrics between wounds. Both subjects and blinded dermatologists preferred the early cosmetic appearance of collagen-treated wounds over primarily closed wounds. Limitations: Small sample size, absence of negative control. Conclusion: These data suggest that collagen powder is non-inferior to primary closure at the macro- and microscopic levels, while possibly leading to superior early cosmetic outcomes and accelerated histologic wound maturation. Ethics/Clinical Trials Registration: Study was approved by the George Washington University Institutional Review Board (IRB protocol #121745). ClinicalTrials.gov: NCT03481907. J Drugs Dermatol. 2019;18(7):667-673.
基于胶原蛋白的产品已应用于伤口愈合，这是由于胶原蛋白具有止血特性、低抗原性以及较差的培养能力。目的：比较局部应用胶原蛋白粉与一期缝合的全层伤口愈合速度和质量。方法：八名志愿者每条大腿接受一次4毫米的打孔活检。一个伤口采用一期缝合处理，另一个伤口每日应用胶原蛋白粉。四周时对伤口进行活检以进行组织病理学分析。受试者在第1、2、4、6和12周对瘙痒、疼痛及治疗偏好进行评分。结果：八处接受胶原蛋白治疗的伤口中有六处在初始受伤后4周完全愈合。伤口的组织学分析显示两组均有表皮再上皮化。在接受胶原蛋白治疗的伤口中观察到更有序的肉芽组织，分别使用Masson三色染色法和CD31染色法对胶原蛋白和新生血管生成进行了确认。受试者报告伤口之间的瘙痒和疼痛指标相似。受试者和不知情的皮肤科医生均更喜欢胶原蛋白治疗伤口而非一期缝合伤口的早期外观。局限性：样本量小，缺乏阴性对照。结论：这些数据表明，在宏观和微观层面，胶原蛋白粉并不逊于一期缝合，同时可能带来更好的早期美容效果并加速组织学伤口成熟。伦理/临床试验注册：该研究获得乔治华盛顿大学机构审查委员会批准（IRB协议编号#121745）。ClinicalTrials.gov：NCT03481907。《药物皮肤病学杂志》。2019年；18(7)：667 - 673。
