Benito-Martínez Selma, Pérez-Köhler Bárbara, Rodríguez Marta, Izco Jesús María, Recalde José Ignacio, Pascual Gemma
Departamento de Medicina y Especialidades Médicas, Facultad de Medicina y Ciencias de la Salud, Universidad de Alcalá, 28805 Alcalá de Henares, Spain.
Biomedical Networking Research Centre of Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), 28029 Madrid, Spain.
Biomedicines. 2022 Apr 21;10(5):960. doi: 10.3390/biomedicines10050960.
Wound healing includes dynamic processes grouped into three overlapping phases: inflammatory, proliferative, and maturation/remodeling. Collagen is a critical component of a healing wound and, due to its properties, is of great interest in regenerative medicine. This preclinical study was designed to compare the effects of a new collagen-based hydrolysate powder on wound repair to a commercial non-hydrolysate product, in a murine model of cutaneous healing. Circular excisional defects were created on the dorsal skin of Wistar rats ( = 36). Three study groups were established according to the treatment administered. Animals were euthanized after 7 and 18 days. Morphometric and morphological studies were performed to evaluate the healing process. The new collagen treatment led to the smallest open wound area throughout most of the study. After seven days, wound morphometry, contraction, and epithelialization were similar in all groups. Treated animals showed reduced granulation tissue formation and fewer inflammatory cells, and induction of vasculature with respect to untreated animals. After 18 days, animals treated with the new collagen treatment showed accelerated wound closure, significantly increased epithelialization, and more organized repair tissue. Our findings suggest that the new collagen treatment, compared to the untreated control group, produces significantly faster wound closure and, at the same time, promotes a slight progression of the reparative process compared with the rest of the groups.
伤口愈合包括动态过程，可分为三个重叠阶段：炎症期、增殖期和成熟/重塑期。胶原蛋白是愈合伤口的关键成分，因其特性，在再生医学中备受关注。本临床前研究旨在比较一种新型胶原蛋白水解产物粉末与市售非水解产物在小鼠皮肤愈合模型中对伤口修复的影响。在Wistar大鼠（n = 36）的背部皮肤制造圆形切除缺损。根据给药治疗建立三个研究组。在7天和18天后对动物实施安乐死。进行形态测量和形态学研究以评估愈合过程。在研究的大部分时间里，新型胶原蛋白治疗导致的开放性伤口面积最小。7天后，所有组的伤口形态测量、收缩和上皮化情况相似。与未治疗的动物相比，接受治疗的动物肉芽组织形成减少，炎症细胞减少，血管生成减少。18天后，接受新型胶原蛋白治疗的动物伤口闭合加速，上皮化显著增加，修复组织更有序。我们的研究结果表明，与未治疗的对照组相比，新型胶原蛋白治疗能显著加快伤口闭合，同时与其他组相比，促进修复过程略有进展。
