Benito-Martínez Selma, Pérez-Köhler Bárbara, Rodríguez Marta, Rivas-Santos Celia, María Izco Jesús, Recalde José Ignacio, Pascual Gemma
Departamento de Medicina y Especialidades Médicas, Facultad de Medicina y Ciencias de la Salud, Universidad de Alcalá, Alcalá de Henares, Madrid, Spain.
Biomedical Networking Research Centre of Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Madrid, Spain.
Int Wound J. 2025 Apr;22(4):e70589. doi: 10.1111/iwj.70589.
Collagen proteins play important roles in wound healing and are of great interest in regenerative medicine. This study evaluated the efficacy of new collagen-based products and compared them to commercial products in a murine model of cutaneous healing. Circular excisional defects were generated on 72 Wistar rats. Six study groups were established according to the administered collagen treatment: Control (without treatment), SD-C (semidenatured), Catrix, Hy-C (hydrolyzed), N-C (native) and Helix3-CP. Seven and eighteen days post-surgery, animals were euthanized. Wound closure was macroscopically assessed by taking zenithal images of the defects. Morphological, histological and immunohistochemical studies were performed to evaluate the healing process. After 7 days, open areas and degree of epithelialization were similar among the groups. Significant differences were observed in contraction between control and the N-C and Helix3-CP groups. Untreated animals exhibited a more pronounced granulation tissue with a high number of inflammatory cells and a disorganised extracellular matrix with type III collagen deposition. After 18 days, animals treated with new collagen (Hy-C and N-C) exhibited accelerated wound closure, increased epithelialization and a more organised granulation tissue. Local administration of new collagen treatments promotes the progression of the reparative process and significantly accelerates wound closure compared with nontreated wounds.
胶原蛋白在伤口愈合中发挥着重要作用，在再生医学领域备受关注。本研究评估了新型胶原蛋白产品的疗效，并在小鼠皮肤愈合模型中将其与市售产品进行比较。在72只Wistar大鼠身上制造圆形切除伤口。根据所给予的胶原蛋白治疗方案设立六个研究组：对照组（未治疗）、SD - C（半变性）、Catrix、Hy - C（水解）、N - C（天然）和Helix3 - CP。术后7天和18天，对动物实施安乐死。通过拍摄伤口顶部图像对伤口闭合情况进行宏观评估。进行形态学、组织学和免疫组织化学研究以评估愈合过程。7天后，各实验组间的伤口开放面积和上皮化程度相似。对照组与N - C组和Helix3 - CP组在伤口收缩方面存在显著差异。未治疗的动物表现出更明显的肉芽组织，伴有大量炎症细胞，以及III型胶原沉积紊乱的细胞外基质。18天后，接受新型胶原蛋白（Hy - C和N - C）治疗的动物伤口闭合加速，上皮化增加，肉芽组织更有序。与未治疗的伤口相比，局部应用新型胶原蛋白治疗可促进修复过程进展并显著加速伤口闭合。
