1 QC Laboratory, Post Harvest Technology, ICAR-Central Institute of Fisheries Education, Versova, Mumbai 400061, India.
2 ICAR-Central Island Agricultural Research Institute, P.O. Box 181, Port Blair 744 101, India.
J Food Prot. 2019 Aug;82(8):1417-1422. doi: 10.4315/0362-028X.JFP-19-101.
Histamine poisoning occurs when temperature-abused marine fish containing elevated levels of histamine are consumed. Histamine-producing bacteria found in fish can colonize processing surfaces and form biofilms. In this study, the biofilm-forming abilities of histamine-producing bacteria from Indian mackerel () and the effect of hypochlorite treatment on biofilm formation were studied. The isolates of this study produced histamine in the range of 471 to 2,126 ppm. The histidine decarboxylase gene was detected in all isolates producing histamine except in one strain each of and . All isolates tested in this study produced moderate biofilms under control conditions, whereas exposure to 1 and 3 ppm of sodium hypochlorite significantly enhanced biofilm formation. However, exposure to 5 ppm of sodium hypochlorite showed an inhibitory effect on biofilm formation by all the isolates except . The results of this study suggest that histamine-producing bacteria can form stable biofilms and that this activity may be enhanced by the application of low levels of sodium hypochlorite, a phenomenon that might influence the persistence of histamine-producing bacteria in fish processing areas.
组胺中毒发生在食用温度滥用的含有高水平组胺的海洋鱼类时。在鱼类中发现的产组胺细菌可以在加工表面定殖并形成生物膜。在这项研究中，研究了印度鲐（）中产组胺细菌的形成生物膜的能力以及次氯酸盐处理对生物膜形成的影响。本研究的分离株在 471 至 2126 ppm 的范围内产生组胺。除了 和 中的一株外，所有产组胺的分离株均检测到组氨酸脱羧酶基因。在本研究中测试的所有分离株在对照条件下均产生中等生物膜，而暴露于 1 和 3 ppm 的次氯酸钠可显著增强生物膜的形成。然而，暴露于 5 ppm 的次氯酸钠除 外，对所有分离株的生物膜形成均表现出抑制作用。这项研究的结果表明，产组胺细菌可以形成稳定的生物膜，并且这种活性可能会被低水平的次氯酸钠增强，这种现象可能会影响产组胺细菌在鱼类加工区域的持久性。
