From the Universidade Anhanguera-UNIDERP (Universidade para o Desenvolvimento do Estado e da Região do Pantanal), Campo Grande, Mato Grosso do Sul, Brazil (PDdOP, JMC); Universidade Estadual de Londrina (UEL) and Universidade do Norte do Paraná (UNOPAR), Londrina, Paraná, Brazil (PDdOP, RCP-F, LDB, FBP); Centro Universitário de Anápolis (UNIEVANGÉLICA), Anápolis, Goiás, Brazil (DAdAP-O, RFdO); and Universidade de Cuiabá (UNIC), Cuiabá, Mato Grosso, Brazil (WS-B, VMS).
Am J Phys Med Rehabil. 2020 Jan;99(1):19-25. doi: 10.1097/PHM.0000000000001265.
The aim of this study was to analyze the effects of low-intensity pulsed ultrasound therapy under different pulse regimes on cultures of semiconfluent L929 fibroblasts, evaluating cell viability, anatomical structural alterations, modulation of vascular endothelial growth factor, interleukin 6, collagen type 1 alpha 1, collagen type 1 alpha 2, and fibroblast growth factor 7, as well as the amount of inflammatory mediators interleukin 2, interleukin 4, interleukin 6, interferon γ, tumor necrosis factor, interleukin 17A, and interleukin 10 at 24, 48, and 72 hrs.
The design was experimental study.
The treatments consisted of 0.2 W/cm doses at a frequency of 1 MHz, with a pulse rate of 10% and 20%. Viability was assessed by the MTT assay (3-(4,5-dimethylthiazole)-2,5-diphenyltetrazolium bromide), gene expression by real-time quantitative polymerase chain reaction, and cytokine quantification by flow cytometry.
At 48 hrs, ultrasound enhanced cell viability and affected interleukin 6 cytokine production, vascular endothelial growth factor, interleukin 6, type 1 alpha 1 and alpha 2 collagens, and fibroblast growth factor 7 gene modulation.
Low-intensity pulsed ultrasound therapy had a biostimulatory effect on semiconfluent in vitro L929 fibroblast cells, where the group with a dose of 0.2 W/cm-10% (G2) presented higher responses, in all the analyzed aspects, toward the dose pulsed to 20%, confirming its therapeutic properties related to the initial phases of tissue healing.
本研究旨在分析不同脉冲模式下低强度脉冲超声治疗对半汇合 L929 成纤维细胞培养物的影响，评估细胞活力、解剖结构改变、血管内皮生长因子、白细胞介素 6、胶原 1α1、胶原 1α2 和成纤维细胞生长因子 7 的调节以及炎症介质白细胞介素 2、白细胞介素 4、白细胞介素 6、干扰素 γ、肿瘤坏死因子、白细胞介素 17A 和白细胞介素 10 的含量，在 24、48 和 72 小时。
该设计为实验研究。
治疗包括 0.2 W/cm 剂量，频率为 1 MHz，脉冲率为 10%和 20%。通过 MTT 测定法（3-（4,5-二甲基噻唑）-2,5-二苯基四唑溴盐）评估细胞活力、实时定量聚合酶链反应评估基因表达、流式细胞术评估细胞因子定量。
在 48 小时时，超声增强了细胞活力并影响了白细胞介素 6 细胞因子的产生、血管内皮生长因子、白细胞介素 6、1α1 和 1α2 胶原以及成纤维细胞生长因子 7 的基因调节。
低强度脉冲超声治疗对半汇合体外 L929 成纤维细胞具有生物刺激作用，其中剂量为 0.2 W/cm-10%（G2）的组在所有分析方面均表现出更高的反应，与 20%脉冲剂量相比，证实了其与组织愈合初始阶段相关的治疗特性。
