Vashakidze R P, Chelidze M G, Mamulashvili N A, Kalandarishvili K G, Tsalkalamanidze N V
Institute of Molecular Biology and Biological Physics, Georgian SSR Academy of Sciences, Tbilisi, USSR.
Nucleic Acids Res. 1988 Jun 10;16(11):4989-94. doi: 10.1093/nar/16.11.4989.
The binding of nuclear proteins from Drosophila melanogaster embryos to simple homopolymeric DNA sequences was studied. Nuclear proteins were electrophoresed, transferred onto nitrocellulose and incubated with labelled synthetic homopolymers or natural fragment containing simple sequences. Several protein bands were found in the 65-72 KDa region, which specifically bind both poly [(dG-dT).(dA-dC)] and a natural fragment containing 40 bp of this sequence. These proteins do not bind to homopolymers poly [(dA).(dT)] and poly [(dG-dA).(dC-dT)], or other foreign DNAs.
研究了黑腹果蝇胚胎中的核蛋白与简单同聚体DNA序列的结合情况。对核蛋白进行电泳，转移到硝酸纤维素膜上，然后与标记的合成同聚物或含有简单序列的天然片段一起孵育。在65 - 72千道尔顿区域发现了几条蛋白带，它们能特异性结合聚[(dG - dT)·(dA - dC)]以及含有该序列40个碱基对的天然片段。这些蛋白不与同聚物聚[(dA)·(dT)]、聚[(dG - dA)·(dC - dT)]或其他外源DNA结合。
