Garreau H, Williams J G
Nucleic Acids Res. 1983 Dec 10;11(23):8473-84. doi: 10.1093/nar/11.23.8473.
Intergenic regions of the Dictyostelium genome contain an extremely high proportion of AT base pairs. Those intergenic regions which have been subjected to nucleotide sequence analysis are predominantly composed of alternating runs of poly(dA) and poly(dT) and there is evidence to suggest that nucleosomes do not form on such sequences. We have identified two nuclear proteins, of molecular weight 70,000 and 74,000 daltons, which bind only to intergenic regions of a cloned Dictyostelium gene. Binding is specifically inhibited in the presence of synthetic poly(dA) - poly (dT) as competitor. These proteins may play some role in the chromosomal organization of intergenic regions in Dictyostelium discoideum.
盘基网柄菌基因组的基因间区域含有极高比例的AT碱基对。那些经过核苷酸序列分析的基因间区域主要由交替排列的聚（dA）和聚（dT）组成，并且有证据表明核小体不会在这些序列上形成。我们已经鉴定出两种分子量分别为70,000和74,000道尔顿的核蛋白，它们仅与克隆的盘基网柄菌基因的基因间区域结合。在存在合成聚（dA）-聚（dT）作为竞争者的情况下，结合被特异性抑制。这些蛋白质可能在盘基网柄菌基因间区域的染色体组织中发挥某种作用。
