Pan Jian-Feng, Yuan Liu, Guo Chang-An, Geng Xiao-Hua, Fei Teng, Fan Wen-Shuai, Li Shuo, Yuan Heng-Feng, Yan Zuo-Qin, Mo Xiu-Mei
Department of Orthopedics, Zhongshan Hospital of Fudan University, 180 Fenglin Road, Shanghai 200032, China.
J Mater Chem B. 2014 Dec 21;2(47):8346-8360. doi: 10.1039/c4tb01221f. Epub 2014 Oct 27.
Hydrogels play a very important role in cartilage tissue engineering. Here, we oxidized dextran (Odex) and modified gelatin (Mgel) to fabricate a fast forming hydrogel without the addition of a chemical crosslinking agent. The dynamic gelling process was measured through rheological measurements. The microstructure was examined by lyophilizing to get porous scaffolds. Biological assessment was performed through CCK-8 assays by using synovium-derived mesenchymal cells (SMSCs) at 1, 3, 7 and 14 days. In vivo evaluation for application in cartilage tissue engineering was performed 8 weeks after subcutaneous injection of SMSC-loaded Odex/Mgel hydrogels combined with TGF-β3 in the dorsa of nude mice. According to the results, a fast forming hydrogel was obtained by simply modifying dextran and gelatin. Moreover, the Odex/Mgel hydrogel exhibited good biocompatibility in cultures of SMSCs and a homogeneous distribution of live cells was achieved inside the hydrogels. After 8 weeks, newly formed cartilage was achieved in the dorsa of nude mice; no inflammatory reaction was observed and high production of GAGs was shown. The method provides a strategy for the design and fabrication of fast in situ forming hydrogels. The Odex/Mgel hydrogel could be used for the regeneration of cartilage in tissue engineering.
水凝胶在软骨组织工程中发挥着非常重要的作用。在此，我们氧化葡聚糖（Odex）并改性明胶（Mgel），以制备一种无需添加化学交联剂的快速成型水凝胶。通过流变学测量来测定动态凝胶化过程。通过冻干获得多孔支架以检查微观结构。在第1、3、7和14天，使用滑膜来源的间充质细胞（SMSC）通过CCK - 8测定法进行生物学评估。在裸鼠背部皮下注射负载SMSC的Odex/Mgel水凝胶与TGF -β3组合8周后，进行软骨组织工程应用的体内评估。根据结果，通过简单地改性葡聚糖和明胶获得了一种快速成型水凝胶。此外，Odex/Mgel水凝胶在SMSC培养物中表现出良好的生物相容性，并且在水凝胶内部实现了活细胞的均匀分布。8周后，在裸鼠背部形成了新的软骨；未观察到炎症反应，并且显示出高含量的糖胺聚糖（GAGs）。该方法为快速原位成型水凝胶的设计和制备提供了一种策略。Odex/Mgel水凝胶可用于组织工程中软骨的再生。
