Hu Ping, Zhao Daoyuan, Zhao Fangzhou, Wang Jing, Zhu Weiyun
National Center for International Research on Animal Gut Nutrition, Jiangsu Key Laboratory of Gastrointestinal Nutrition and Animal Health, Laboratory of Gastrointestinal Microbiology, National Experimental Teaching Demonstration Center of Animal Science, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
College of Science, Nanjing Agricultural University, Nanjing 210095, China.
Animals (Basel). 2019 Jul 11;9(7):438. doi: 10.3390/ani9070438.
Iron deficiency is considered a common nutritional problem for suckling piglets. The aim of this study was to evaluate the effects of the combination of oral lactoferrin and iron injection on iron levels, antioxidant ability and cytokine activity in suckling piglets. A total of sixty suckling piglets taken from six sows (10 piglets per litter) with a similar parity were chosen. The lactoferrin (LF) group was orally administrated with lactoferrin solution (0.5 g/kg body weight per day) for a week, the CON group was orally administrated with the same dose of physiological saline. Each piglet (all groups) was given 100 mg of iron dextran (FeDex) by intramuscular injection at the third day of age. Six piglets (n = 6) from each group were euthanized on days 8 and 21. The oral lactoferrin improved the iron level of suckling piglets by increasing the concentrations of serum hemoglobin and hepatic iron on day 8. Gene expression of lactoferrin receptor (LFR) was significantly increased in the LF group piglets on day 8, while duodenal protein expression of the divalent metal transporter 1 (DMT1) was significantly reduced in the LF group on day 8. In addition, oral lactoferrin enhanced serum T-AOC activities and duodenal SOD activities on day 21. The LF piglets had a significantly increased serum concentration of IL-10 on day 8. These results indicated that a combination of oral lactoferrin and iron injection is a more effective method of improving the iron level by up-regulating the expression of the LFR gene, enhancing the antioxidant ability and modulating the cytokine activity in the suckling piglets.
缺铁被认为是哺乳仔猪常见的营养问题。本研究的目的是评估口服乳铁蛋白和注射铁剂联合使用对哺乳仔猪铁水平、抗氧化能力和细胞因子活性的影响。总共从六头胎次相近的母猪中选取了60头哺乳仔猪（每窝10头仔猪）。乳铁蛋白（LF）组口服乳铁蛋白溶液（每天0.5 g/kg体重），持续一周，CON组口服相同剂量的生理盐水。每头仔猪（所有组）在3日龄时通过肌肉注射给予100 mg右旋糖酐铁（FeDex）。在第8天和第21天，每组处死6头仔猪（n = 6）。口服乳铁蛋白通过提高第8天血清血红蛋白和肝脏铁的浓度，改善了哺乳仔猪的铁水平。第8天，LF组仔猪乳铁蛋白受体（LFR）的基因表达显著增加，而LF组第8天十二指肠中二价金属转运体1（DMT1）的蛋白表达显著降低。此外，口服乳铁蛋白在第21天增强了血清总抗氧化能力（T-AOC）活性和十二指肠超氧化物歧化酶（SOD）活性。LF组仔猪在第8天血清白细胞介素-10（IL-10）浓度显著升高。这些结果表明，口服乳铁蛋白和注射铁剂联合使用是一种更有效的方法，可通过上调LFR基因表达、增强抗氧化能力和调节哺乳仔猪的细胞因子活性来提高铁水平。
